Cas no 53-57-6 (NADPH)

β-NADPH-d4 is a deuterium-labeled form of β-nicotinamide adenine dinucleotide phosphate (NADPH), where four hydrogen atoms are replaced with deuterium. This isotopically labeled compound is widely used as an internal standard in quantitative mass spectrometry (MS) and nuclear magnetic resonance (NMR) studies, ensuring high precision in metabolic and enzymatic research. Its structural similarity to native NADPH minimizes interference while providing distinct detection signals. β-NADPH-d4 is particularly valuable in tracing redox reactions, studying enzyme kinetics, and investigating cellular metabolism pathways. The deuterium labeling enhances stability and reduces metabolic scrambling, making it a reliable tool for accurate quantification in complex biological matrices.
NADPH structure
NADPH structure
Product Name:NADPH
CAS No:53-57-6
MF:C21H30N7O17P3
MW:745.420887470245
CID:371676
PubChem ID:5884
Update Time:2025-12-25

NADPH Chemical and Physical Properties

Names and Identifiers

    • [[(2S,3S,4S,5S)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxy-te trahydrofuran-2-yl]methoxy-hydroxy-phosphoryl] [(2S,3R,4S,5S)-5-( 3-carbamoyl-4H-pyridin-1-yl)-3,4-dihydroxy-tetrahydrofuran-2-yl]m et
    • Adenosine5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), P'&reg
    • β-NADPH-d4
    • 5'-ester with 1,4-dihydro-1-b-D-ribofuranosyl-3-pyri
    • 5'-ester with 1,4-dihydro-1-b-D-ribofuranosyl-3-pyridinecarboxamide
    • Di-(tert-butyl) imidodicarbonate
    • Di-t-butyl iminodicarboxylate
    • di-tert-butyl imidodicarbonate
    • di-tert-butyl iminodicarbonate
    • Di-tert-butyl Iminodicarboxylate
    • Di-tert-butyl-iminodicarboxylate
    • Iminodicarboxylic Acid Di-tert-butyl Ester
    • N-Boc-tert-butylcarbamate
    • tert-Butyl iminodicarboxylate
    • β-TPNH
    • β-Nicotinamide-adenine-dinucleotide-phosphoric acid
    • β-NADPH
    • Triphosphopyridine nucleotide, reduced
    • TPNH
    • Reduced triphosphopyridine nucleotide
    • Reduced nicotinamide adenine dinucleotide phosphate
    • Reduced 2 codehydrogenase II
    • Nicotinamide-adenine dinucleotide phosphate, reduced
    • NADPH
    • Dihydrocodehydrogenase II
    • Cozymase II, reduced
    • Coenzyme II, reduced
    • Codehydrogenase II, reduced
    • Codehydrase II, reduced
    • 51: PN: WO2004076659 FIGURE: 7 claimed sequence
    • Adenosine, 2'-(dihydrogen phosphate) 5'-(trihydrogen pyrophosphate), 5'→5'-ester with 1,4-dihydro-1-β-D-ribofuranosylnicotinamide (8CI)
    • Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), P'→5'-ester with 1,4-dihydro-1-β-D-ribofuranosyl-3-pyridinecarboxamide
    • 53-57-6
    • b-Nicotinamide-adenine-dinucleotide-phosphorate
    • Dihydrocodehydrogenase II
    • Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), P'-5'-ester with 1,4-dihydro-1-beta-D-ribofuranosyl-3-pyridinecarboxamide
    • C21-H30-N7-O17-P3.4Na
    • NADP REDUCED FORM [MI]
    • ACFIXJIJDZMPPO-NNYOXOHSSA-N
    • DTXSID001018921
    • beta-Nicotinamide-adenine-dinucleotide-phosphoric acid
    • C00005
    • C21H30N7O17P3
    • DB02338
    • Nicotinamide-adenine-dinucleotide-phosphorate
    • CHEBI:16474
    • EINECS 200-177-6
    • ADENOSINE 5'-(TRIHYDROGEN DIPHOSPHATE), 2'-(DIHYDROGEN PHOSPHATE), P'->5'-ESTER WITH 1,4-DIHYDRO-1-.BETA.-D-RIBOFURANOSYL-3-PYRIDINECARBOXAMIDE
    • beta-Nicotinamide-adenine-dinucleotide-phosphorate
    • NADP REDUCED FOM
    • C21-H30-N7-O17-P3
    • Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), P'.fwdarw.5'-ester with 1,4-dihydro-1-.beta.-D-ribofuranosyl-3-pyridinecarboxamide
    • reduced Cozymase II
    • beta-NADPH Tetrasodium
    • CHEMBL407009
    • Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), 5'.fwdarw.5'-ester with 1,4-dihydro-1-.beta.-D-ribofuranosyl-3-pyridinecarboxamide
    • Reduced Nicotinamide Adenine Dinucleotide Phosphate
    • Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), P'?5'-ester with 1,4-dihydro-1-.beta.-D-ribofuranosyl-3-pyridinecarboxamide
    • reduced nicotinamide-adenine dinucleotide phosphate
    • REDUCED CODEHYDROGENASE II
    • COENZYME II, REDUCED
    • dihydrotriphosphopyridine nucleotide reduced
    • NADPH
    • Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogenphosphate), P'-5'-ester with 1,4-dihydro-1-beta-D-ribofuranosyl-3-pyridinecarboxamide
    • Reduced triphosphopyridine nucleotide
    • reducednicotinamide-adenine dinucleotide phosphate
    • NADPH2
    • reduced Coenzyme II
    • .BETA.-NADPH
    • 2646-71-1
    • Nicotinamide-adenine-dinucleotide-phosphoric acid
    • 2'-(dihydrogen phosphate) 5'-(trihydrogen pyrophosphate) Adenosine 5'-ester with 1,4-dihydro-1-b-D-ribofuranosylnicotinamide
    • dihydronicotinamide adenine dinucleotide-P
    • Adenosine 5'-(trihydrogen diphosphate) 2'-(dihydrogen phosphate) P'-5'-ester with 1,4-dihydro-1-beta-delta-ribofuranosyl-3-pyridinecarboxamide
    • TPNH
    • Nicotinamide-adenine dinucleotide phosphate, reduced
    • NADP-reduced
    • b-NADPH
    • Triphosphopyridine nucleotide reduced
    • .BETA.-NICOTINAMIDE-ADENINE-DINUCLEOTIDE-PHOSPHORIC ACID
    • Adenosine 5'-(trihydrogen diphosphate) 2'-(dihydrogen phosphate) P'-5'-ester with 1,4-dihydro-1-beta-D-ribofuranosyl-3-pyridinecarboxamide
    • [[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2R,3S,4R,5R)-5-(3-carbamoyl-4H-pyridin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl hydrogen phosphate
    • 2'-O-phosphonoadenosine 5'-{3-[1-(3-carbamoyl-1,4-dihydropyridin-1-yl)-1,4-anhydro-D-ribitol-5-yl] dihydrogen diphosphate}
    • beta-NADPH
    • dihydronicotinamide adenine dinucleotide phosphate
    • bmse000055
    • nadph hydride
    • b-Nicotinamide-adenine-dinucleotide-phosphoric acid
    • 2'-(dihydrogen phosphate) 5'-(trihydrogen pyrophosphate) Adenosine 5'-ester with 1,4-dihydro-1-beta-delta-ribofuranosylnicotinamide
    • 381Q4X082D
    • Nicotinamide adenine dinucleotide phosphate - reduced
    • Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), P'->5'-ester with 1,4-dihydro-1-beta-D-ribofuranosyl-3-pyridinecarboxamide
    • reduced Codehydrase II
    • UNII-381Q4X082D
    • Q26841327
    • [(2R,3R,4R,5R)-2-(6-Aminopurin-9-yl)-5-[[[[(2R,3S,4R,5R)-5-(3-carbamoyl-4H-pyridin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxymethyl]-4-hydroxy-oxolan-3-yl]oxyphosphonic acid
    • SCHEMBL2041
    • C21H30N7O17P3.4Na
    • Dihydronicotinamide-adenine dinucleotide phosphate
    • Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), P'.fwdarw.5'-ester with 1,4-dihydro-1-beta-D-ribofuranosyl-3-pyridinecarboxamide
    • Adenosine, 2'-(dihydrogen phosphate) 5'-(trihydrogen pyrophosphate), 5'→5'-ester with 1,4-dihydro-1-β-D-ribofuranosylnicotinamide (8CI)
    • 51: PN: WO2004076659 FIGURE: 7 claimed sequence
    • Codehydrase II, reduced
    • Codehydrogenase II, reduced
    • Cozymase II, reduced
    • Triphosphopyridine nucleotide, reduced
    • β-NADPH
    • β-Nicotinamide-adenine-dinucleotide-phosphoric acid
    • β-TPNH
    • NS00015230
    • NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
    • NADPH tetraanion
    • Dihydronicotinamide-adenine dinucleotide phosphoric acid
    • (((2S,3S,4S,5S)-2-(6-amino-9H-purin-9-yl)-5-((((((((2S,3R,4S,5S)-5-(3-carbamoyl-1,4-dihydropyridin-1-yl)-3,4-dihydroxyoxolan-2-yl)methoxy)(hydroxy)phosphoryl)oxy)(hydroxy)phosphoryl)oxy)methyl)-4-hydroxyoxolan-3-yl)oxy)phosphonic acid
    • NADPH dihydro-nicotinamide-adenine-dinucleotide phosphoric acid
    • HY-113324
    • [[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2R,3S,4R,5R)-5-(3-carbamoyl-1,4-dihydropyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate
    • DA-55982
    • NADP-red
    • [(2S,3S,4S,5S)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methyl [[(2S,3R,4S,5S)-5-(3-carbamoyl-4H-pyridin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] hydrogen phosphate
    • Nucleotide, Triphosphopyridine
    • 25 MG -NICOTINAMIDE ADENINE DINUCLEOTIDEPHOSPHATE REDUCED.NA4-SALT AN.GR.
    • Reduced nicotinamide adenine dinucleotide phosphoric acid
    • Adenosine 5'(trihydrogen diphosphate), 2'(dihydrogen phosphate), P'.fwdarw.5'ester with 1,4dihydro1betaDribofuranosyl3pyridinecarboxamide
    • (((2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl)methoxy-hydroxyphosphoryl) ((2R,3S,4R,5R)-5-(3-carbamoyl-1,4-dihydropyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl)methyl phosphate
    • beta-TPNH
    • Reduced nicotinamide-adenine dinucleotide phosphoric acid
    • reduced NADP
    • Dihydronicotinamideadenine dinucleotide phosphate
    • {[(2S,3S,4S,5S)-2-(6-amino-9H-purin-9-yl)-5-[({[({[(2S,3R,4S,5S)-5-(3-carbamoyl-1,4-dihydropyridin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}(hydroxy)phosphoryl)oxy](hydroxy)phosphoryl}oxy)methyl]-4-hydroxyoxolan-3-yl]oxy}phosphonic acid
    • (((2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl)methoxy-hydroxyphosphoryl) ((2R,3S,4R,5R)-5-(3-carbamoyl-4H-pyridin-1-yl)-3,4-dihydroxyoxolan-2-yl)methyl hydrogen phosphate
    • DTXCID201476935
    • dihydronicotinamide adenine dinucleotide phosphate reduced
    • Adenosine 5'(trihydrogen diphosphate), 2'(dihydrogen phosphate), P'>5'ester with 1,4dihydro1betaDribofuranosyl3pyridinecarboxamide
    • dihydrotriphosphopyridine nucleotide
    • NADP REDUCED FORM
    • 2'-O-phosphonoadenosine 5'-(3-(1-(3-carbamoyl-1,4-dihydropyridin-1-yl)-1,4-anhydro-D-ribitol-5-yl) dihydrogen diphosphate)
    • Phosphate, Nicotinamide-Adenine Dinucleotide
    • ((2S,3S,4S,5S)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl)methyl (((2S,3R,4S,5S)-5-(3-carbamoyl-4H-pyridin-1-yl)-3,4-dihydroxyoxolan-2-yl)methoxy-hydroxyphosphoryl) hydrogen phosphate
    • CS-0059609
    • Dinucleotide Phosphate, Nicotinamide-Adenine
    • Nadph dihydro-nicotinamide-adenine-dinucleotidephosphoric acid
    • reduced dihydrotriphosphopyridine nucleotide
    • Inchi： 1S/C21H30N7O17P3/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(44-46(33,34)35)14(30)11(43-21)6-41-48(38,39)45-47(36,37)40-5-10-13(29)15(31)20(42-10)27-3-1-2-9(4-27)18(23)32/h1,3-4,7-8,10-11,13-16,20-21,29-31H,2,5-6H2,(H2,23,32)(H,36,37)(H,38,39)(H2,22,24,25)(H2,33,34,35)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1
    • InChI Key： ACFIXJIJDZMPPO-NNYOXOHSSA-N
    • SMILES： P(=O)(O)(O)O[C@H]1[C@H](N2C=NC3C(N)=NC=NC2=3)O[C@H](COP(=O)(O)OP(=O)(O)OC[C@@H]2[C@H]([C@H]([C@H](N3C=CCC(C(N)=O)=C3)O2)O)O)[C@H]1O

Computed Properties

  • Exact Mass: 745.091102
  • Monoisotopic Mass: 745.091102
  • Isotope Atom Count: 0
  • Hydrogen Bond Donor Count: 9
  • Hydrogen Bond Acceptor Count: 18
  • Heavy Atom Count: 48
  • Rotatable Bond Count: 13
  • Complexity: 1410
  • Covalently-Bonded Unit Count: 1
  • Defined Atom Stereocenter Count: 8
  • Undefined Atom Stereocenter Count : 0
  • Defined Bond Stereocenter Count: 0
  • Undefined Bond Stereocenter Count: 0
  • XLogP3: _6.8
  • Topological Polar Surface Area: 364

Experimental Properties

  • Density: 2.28
  • Boiling Point: 1175.1°Cat760mmHg
  • Flash Point: 664.5°C
  • Refractive Index: 1.849
  • PSA: 394.57000
  • LogP: -0.90060

NADPH Pricemore >>

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NADPH Production Method

Production Method 1

Reaction Conditions
1.1R:i-PrNH2, S:H2O, 8 h, 25°C, pH 8
Reference
Transforming inert cycloalkanes into α,ω-diamines by designed enzymic cascade catalysis
By Zhang, Zhongwei et al, Angewandte Chemie, 2023, 62(16), e202215935

Production Method 2

Reaction Conditions
1.1R:HCO2- ?Na+, R:(NH4)2SO4, C:1449222-31-4, S:H2O, 1.5 h, < 40°C, pH 7.4; 1 h, < 40°C, pH 7.4
1.2R:PhNCS, R:Et3N, S:MeCN, 1 h, rt
Reference
The visible-light-driven transfer hydrogenation of nicotinamide cofactors with a robust ruthenium complex photocatalyst
By Dong, Wenjin et al, Green Chemistry, 2020, 22(7), 2279-2287

Production Method 3

Reaction Conditions
1.1R:N(CH2CH2OH)3, C:1366596-70-4 (reaction product with carboxy-functionaliz), C:121596-51-8, S:H2O, S:DMF, 150 min, rt, pH 7
Reference
A Photocatalyst/Enzyme Couple That Uses Solar Energy in the Asymmetric Reduction of Acetophenones
By Choudhury, Sumit et al, Angewandte Chemie, 2012, 51(46), 11624-11628

Production Method 4

Reaction Conditions
1.1C:9028-12-0, C:9028-53-9, S:H2O, S:DMSO, 4 h, 30°C, pH 7.5
Reference
Application of biocatalysis towards asymmetric reduction and hydrolytic desymmetrisation in the synthesis of a β-3 receptor agonist
By Badland, Matthew et al, Green Chemistry, 2011, 13(10), 2888-2894

Production Method 5

Reaction Conditions
1.1R:Cleland's reagent, R:N(CH2CH2OH)3, R:NaOD, S:H2O, 4 h, rt, pH 8.9
Reference
Cofactor mobility determines reaction outcome in the IMPDH and GMPR (β-α)8 barrel enzymes
By Patton, Gregory C. et al, Nature Chemical Biology, 2011, 7(12), 950-958

Production Method 6

Reaction Conditions
1.1R:Zn (myoglobin complex), R:N(CH2CH2OH)3, 3.5 h, rt, pH 9; 7 d, rt, pH 9
Reference
Phototriggered chemical reduction of NADP+ by Zn-reconstituted myoglobin and triethanolamine as a sacrifical donor
By Nishiyama, Katsuhiko et al, Chemistry Letters, 2005, 34(7), 1032-1033

Production Method 7

Reaction Conditions
1.1R:H2, C:9027-05-8, S:H2O, 2 h, 40°C, 5 bar, pH 8
Reference
Membrane aerated hydrogenation: Enzymatic and chemical homogeneous catalysis
By Greiner, Lasse et al, Advanced Synthesis & Catalysis, 2003, 345(6+7), 679-683

Production Method 8

Reaction Conditions
1.1R:H2, C:9027-05-8 (immobilized), 80°C
1.2S:H2O, 40°C, pH 8.5
Reference
Utilization of adsorption effects for the continuous reduction of NADP+ with molecular hydrogen by Pyrococcus furiosus hydrogenase
By Greiner, L. et al, Green Chemistry, 2003, 5(6), 697-700

Production Method 9

Reaction Conditions
1.1
Reference
NADPH manufacture using permeabilized microorganisms
By Silhankova, Ludmila and Hajek, Petr, Czech., From Czech., 266763, 12 Jan 1990, 266763, 12 Jan 1990

Production Method 10

Reaction Conditions
1.1R:C:15086-94-9 (reaction products with aminomethylpolystyr), C:2920852-50-0, S:H2O
Reference
Polystyrene-based eosin-Y as a photocatalyst for solar light-mediated NADH/NADPH regeneration and organic transformations
By Singh, Pooja et al, Reaction Chemistry & Engineering, 2023, 8(5), 1072-1082

Production Method 11

Reaction Conditions
1.1C:CdS
Reference
Protein-Mediated Biosynthesis of Semiconductor Nanocrystals for Photocatalytic NAD(P)H Regeneration and Chiral Amine Production
By Bachar, Oren et al, Angewandte Chemie, 2022, 61(23), e202202457

Production Method 12

Reaction Conditions
1.1R:HCl, S:H2O, 10 min
Reference
An Implantable Ionic Wireless Power Transfer System Facilitating Electrosynthesis
By Kim, Chong-Chan et al, ACS Nano, 2020, 14(9), 11743-11752

Production Method 13

Reaction Conditions
1.1
Reference
Development of an enzymatic process for the synthesis of (S)-2-chloro-1-(2,4-dichlorophenyl) ethanol
By Wei, Teng-Yun et al, Organic Process Research & Development, 2019, 23(9), 1822-1828

Production Method 14

Reaction Conditions
1.1R:MgCl2, R:Na2HPO4, R:NH4Cl, R:NaCl, R:MgSO4, R:KH2PO4, S:H2O, 37°C
Reference
Co-production of hydrogen and ethanol by pfkA-deficient Escherichia coli with activated pentose-phosphate pathway: reduction of pyruvate accumulation
By Sekar, Balaji Sundara et al, Biotechnology for Biofuels, 2016, 9, 95/1-95/11

Production Method 15

Reaction Conditions
1.1R:Me2CHOH, S:H2O, 30 min, 40°C, pH 9.0
Reference
Contribution to catalysis of ornithine binding residues in ornithine N5-monooxygenase
By Robinson, Reeder et al, Archives of Biochemistry and Biophysics, 2015, 585, 25-31

Production Method 16

Reaction Conditions
1.1C:9029-33-8, S:H2O, 6°C, pH 8.0
Reference
Structural backgrounds for the formation of a catalytically competent complex with NADP(H) during hydride transfer in ferredoxin-NADP+ reductases
By Sanchez-Azqueta, Ana et al, Biochimica et Biophysica Acta, 2012, 1817(7), 1063-1071

Production Method 17

Reaction Conditions
1.1R:Cleland's reagent, R:MgCl2, R:R:R:R:R:R:H2O, R:KOH, R:NH4Cl, C:56-65-5, C:Coenzyme II, C:9001-51-8, C:9001-40-5, C:9073-95-4, C:9023-83-0, C:9015-83-2, C:9026-23-7, C:9012-49-1, C:9024-93-5, C:9029-03-2, C:9030-25-5, C:9024-62-8, C:37278-21-0, C:9013-02-9, C:9029-12-3, C:9001-15-4, C:1927-31-7, S:H2O
Reference
Enzymatic De Novo Pyrimidine Nucleotide Synthesis
By Schultheisz, Heather L. et al, Journal of the American Chemical Society, 2011, 133(2), 297-304

Production Method 18

Reaction Conditions
1.1S:872672-50-9, S:H2O, pH 7
Reference
Ionic Liquids as Performance Additives for Electroenzymatic Syntheses
By Kohlmann, Christina et al, Chemistry - A European Journal, 2009, 11692-11700, S11692/1-S11692/11

Production Method 19

Reaction Conditions
1.1C:121596-52-9, S:H2O, pH 7
Reference
Synthesis, characterization and application of new rhodium complexes for indirect electrochemical cofactor regeneration
By Hildebrand, Falk et al, Advanced Synthesis & Catalysis, 2008, 350(6), 909-918

Production Method 20

Reaction Conditions
1.1R:D-Glucose, C:9028-53-9
Reference
α-Secondary Isotope Effects as Probes of "Tunneling-Ready" Configurations in Enzymatic H-Tunneling: Insight from Environmentally Coupled Tunneling Models
By Pudney, Christopher R. et al, Journal of the American Chemical Society, 2006, 128(43), 14053-14058

Production Method 21

Reaction Conditions
1.1R:Tris buffer, R:H2, C:9027-05-8, S:774-48-1, 1 h, 40°C, 2.3 bar, pH 8
Reference
Reaction engineering aspects of enzymic manufacture of NADPH in an enzyme membrane reactor
By Mertens, Rita et al, Chemie Ingenieur Technik, 2005, 77(5), 609-616

Production Method 22

Reaction Conditions
1.1C:160261-98-3, S:H2O, 20-25°C, pH 8
Reference
Indirect electrochemical reduction of nicotinamide coenzymes
By Vuorilehto, K. et al, Bioelectrochemistry, 2004, 65(1), 1-7

Production Method 23

Reaction Conditions
1.1R:H2, C:142903-68-2, S:H2O, 3 h, 40°C, 70 psi, pH 8.3
Reference
Transition-Metal-Catalyzed Regeneration of Nicotinamide Coenzymes with Hydrogen
By Wagenknecht, Paul S. et al, Organometallics, 2003, 22(6), 1180-1182

NADPH Raw materials

NADPH Preparation Products

NADPH Suppliers

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(CAS:53-57-6)NADPH
Order Number:NC21065
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Quantity:10g
Purity:97%
Pricing Information Last Updated:Friday, 18 July 2025 16:05
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NADPH Related Literature

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Additional information on NADPH

Beta-NADPH-d4: A Comprehensive Overview

Beta-NADPH-d4, also known as β-NADPH-d4, is a deuterated derivative of β-nicotinamide adenine dinucleotide phosphate (β-NADPH). With the CAS number 53-57-6, this compound plays a crucial role in various biochemical processes, particularly in cellular metabolism and redox reactions. The deuterium labeling in β-NADPH-d4 makes it an invaluable tool in isotopic studies, enabling researchers to track metabolic pathways with unparalleled precision.

The structure of β-NADPH-d4 consists of a nicotinamide group, a ribose sugar, and an adenine nucleotide, with the addition of four deuterium atoms. This deuterium substitution enhances the compound's stability and provides a unique signature for analytical techniques such as mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy. Recent advancements in isotopic labeling techniques have further expanded the applications of β-NADPH-d4 in metabolic flux analysis and enzyme kinetics studies.

In cellular biology, β-NADPH is a key cofactor in biosynthetic reactions and acts as a reducing agent in processes like fatty acid synthesis and glutathione production. The d4 variant allows researchers to distinguish between endogenous and exogenous NADPH pools, which is critical for understanding cellular homeostasis. For instance, studies using β-NADPH-d4 have shed light on the interplay between glycolysis and the pentose phosphate pathway in cancer cells, revealing novel therapeutic targets.

From a biochemical standpoint, β-NADPH-d4 is often utilized in assays to measure enzyme activity, particularly for enzymes such as glucose-6-phosphate dehydrogenase (G6PD) and malic enzyme. These assays are essential for diagnosing metabolic disorders and evaluating the efficacy of potential drug candidates. Recent research has also explored the use of β-NADPH-d4 in studying redox signaling pathways, highlighting its role in inflammation and oxidative stress responses.

In terms of applications, β-NADPH-d4 is widely used in academia and industry for metabolomics studies, drug discovery, and basic research. Its ability to serve as a tracer in complex biological systems makes it indispensable for understanding metabolic networks. Furthermore, advancements in mass spectrometry have enabled high-resolution detection of β-NADPH-d4, facilitating its use in large-scale metabolomic analyses.

Looking ahead, the demand for β-NADPH-d4 is expected to grow as researchers continue to unravel the complexities of cellular metabolism. Its role in personalized medicine and precision healthcare is particularly promising, with potential applications in disease modeling and therapeutic development. As technology evolves, so too will the capabilities of this versatile compound.

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NewCan Biotech Limited
(CAS:53-57-6)NADPH
NC21065
Purity:97%
Quantity:10g
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