An immunochromatographic strip sensor for sildenafil and its analogues?
Journal of Materials Chemistry B Pub Date: 2019-03-09 DOI: 10.1039/C9TB00280D
Abstract
In this study, a new hapten of sildenafil (Sild) was successfully synthesized and a sensitive monoclonal antibody (mAb) against Sild was produced based on this new hapten. The subclass of the mAb was IgG2a, and the half-maximal inhibitory concentration (IC50) of the mAb was 0.53 ng mL?1. Next, an immunochromatographic assay (ICA) was established for detecting Sild and its analogues in functional foods, where the visual detection limit (vLOD) and cut-off values were 0.5 and 20 μg kg?1, respectively. With the aid of a strip scan reader, the ICA can measure Sild with an LOD of 0.7 μg kg?1 and a line range of detection between 1.40 and 13.11 μg kg?1. The whole test process takes only 15 min. Therefore, the ICA provides a useful tool for the on-site detection and rapid initial screening of Sild in functional food samples.
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Journal Name：Journal of Materials Chemistry B
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CAS no.: 89640-58-4
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