Production Method 1

638-07-3

90866-33-4

Reaction Conditions

1.1 Reagents: Glucose ,  NADP Catalysts: Glucose dehydrogenase ,  Reductase Solvents: Water ;  180 min, pH 7.5, 30 °C
1.2 Reagents: Sodium hydroxide Solvents: Water ;  pH 7 - 7.5, 30 °C

Reference

Enantioselective bioconversion using Escherichia coli cells expressing Saccharomyces cerevisiae reductase and Bacillus subtilis glucose dehydrogenase

Park, Hyun Joo; Jung, Jihye; Choi, Hyejeong; Uhm, Ki-Nam; Kim, Hyung-Kwoun, Journal of Microbiology and Biotechnology, 2010, 20(9), 1300-1306

Production Method 2

84367-31-7

90866-33-4

Reaction Conditions

1.1 Reagents: Hydrochloric acid Solvents: Ethanol ;  8 - 10 h, 60 °C

Reference

pH-controlled regioselective nucleophilic ring-opening of epoxide: an improved process for the preparation of (R)-(-)- or (S)-(+)-3-hydroxytetrahydrofuran

Chakraborty, Ankita; Shiva Krishna, Avula; Sheelu, Gurrala; Ghosh, Subhash; Kumaraguru, Thenkrishnan, Organic & Biomolecular Chemistry, 2022, 20(34), 6863-6868

Production Method 3

638-07-3

90866-33-4

Reaction Conditions

1.1 Reagents: Glucose Catalysts: Glucose 6-phosphate dehydrogenase ,  NADP-dependent alc. dehydrogenase Solvents: Water ;  pH 6, 32 °C

Reference

Asymmetric reduction of ethyl 4-chloroacetoacetate to R-(+)-4-chloro-3-hydroxybutyrate by recombinant Escherichia coli strains

Jing, Li; Lin, Jianping; Xu, Zhinan; Tan, Tianwei; Cen, Peilin, Huaxue Fanying Gongcheng Yu Gongyi, 2006, 22(4), 350-355

Production Method 4

638-07-3

90866-33-4

86728-85-0

Reaction Conditions

1.1 Reagents: Glucose ,  NADP ,  NAD ,  Glucose 6-phosphate ,  Isopropanol ,  Allyl bromide ,  Sodium hydroxide ,  Sodium phosphate ,  Magnesium chloride Catalysts: Glucose 6-phosphate dehydrogenase ,  Hexokinase ,  Alcohol dehydrogenase Solvents: Butyl acetate ,  Water ;  1 h, pH 7.0, 30 °C; 4 h, pH 7.0, 30 °C; 6 h, pH 7.0, 30 °C

Reference

Bioconversion of ethyl 4-chloro-3-oxobutanoate by permeabilized fresh brewer's yeast cells in the presence of allyl bromide

Yu, Ming-An; et al, Journal of Industrial Microbiology & Biotechnology, 2007, 34(2), 151-156

Production Method 5

638-07-3

90866-33-4

86728-85-0

Reaction Conditions

1.1 Reagents: Glucose ,  NADP ,  Sodium carbonate Catalysts: Copper ;  pH 7, 30 °C

Reference

Highly efficient synthesis of pharmaceutically relevant chiral 3-N-substituted-azacyclic alcohols using two enantiocomplementary short chain dehydrogenases

Yang, Lin; et al, Biochemical Engineering Journal, 2022, 178,

Production Method 6

638-07-3

90866-33-4

Reaction Conditions

1.1 Reagents: Glucose ,  L-Glutamine ,  (+)-Xylose ,  β-Cyclodextrin Solvents: Butyl acetate ,  Water ;  24 h, pH 6.5, 30 °C
1.2 Reagents: Sodium hydroxide Solvents: Water ;  pH 6.5

Reference

Effective biosynthesis of ethyl (R)-4-chloro-3-hydroxybutanoate by supplementation of L-glutamine, D-xylose and β-cyclodextrin in n-butyl acetate-water media

He, Yu-Cai; Tao, Zhi-Cheng; Ding, Yun; Zhang, Dan-Ping; Wu, Yin-Qin; et al, Journal of Biotechnology, 2015, 203, 62-67

Production Method 7

638-07-3

90866-33-4

Reaction Conditions

1.1 Reagents: NADPH ,  2-(1-Oxopropoxy)acetaldehyde Catalysts: Aldo-keto reductase Solvents: Water ;  12 h, pH 7, 50 °C

Reference

Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity

Ni, Yan; Li, Chun-Xiu; Ma, Hong-Min; Zhang, Jie; Xu, Jian-He, Applied Microbiology and Biotechnology, 2011, 89(4), 1111-1118

Production Method 8

638-07-3

90866-33-4

Reaction Conditions

1.1 Reagents: Glucose ,  NADPH Solvents: Water ;  7 h, pH 6.0, rt

Reference

Efficient production of recombinant aldehyde reductase and its application for asymmetric reduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate

Jing, Keju; Xu, Zhinan; Liu, Ying; Jiang, Xiaoxia; Peng, Li; et al, Preparative Biochemistry & Biotechnology, 2005, 35(3), 203-215

Production Method 9

638-07-3

90866-33-4

Reaction Conditions

1.1 Reagents: Hydrogen Catalysts: Ruthenium trichloride ,  (R)-MeO-BIPHEP Solvents: Methanol

Reference

Asymmetric hydrogenation reactions using a practical in situ generation of chiral ruthenium-diphosphine catalysts from anhydrous RuCl3

Madec, J.; Pfister, X.; Phansavath, P.; Ratovelomanana-Vidal, V.; Genet, J.-P., Tetrahedron, 2001, 57(13), 2563-2568

Production Method 10

638-07-3

90866-33-4

Reaction Conditions

1.1 Solvents: Isopropanol ,  Water ,  Glycine, L-γ-glutamyl-L-cysteinyl-, compd. with 2-hydroxy-N,N,N-trimethylethanam… ;  2 h, pH 7.5, 30 °C
1.2 Reagents: Ethyl acetate

Reference

Efficient asymmetric synthesis of ethyl (R)-3-hydroxybutyrate by recombinant Escherichia coli cells under high substrate loading using eco-friendly ionic liquids as cosolvent

Duan, Zhiwen; Wang, Yaowu; Ouyang, Bin; Wang, Pu, Bioprocess and Biosystems Engineering, 2023, 46(9), 1293-1302

Production Method 11

638-07-3

90866-33-4

Reaction Conditions

1.1 Catalysts: NADPH ,  Glucose dehydrogenase ,  Reductase Solvents: Water

Reference

Asymmetric reduction of β-keto esters with an enzyme from bakers' yeast

Kawai, Yasushi; Tsujimoto, Munekazu; Kondo, Shinichi; Takanobe, Kousuke; Nakamura, Kaoru; et al, Bulletin of the Chemical Society of Japan, 1994, 67(2), 524-8

Production Method 12

638-07-3

90866-33-4

Reaction Conditions

1.1 Reagents: NADP Catalysts: Iron oxide (Fe3O4) (supported on helical multi-walled carbon nanotubes) ,  Carbon ,  Aldo-keto reductase ;  8 h, 35 °C

Reference

Chemoenzymatic catalysis of tert-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate by aldo-keto reductase coupled with composite Fe3O4 nanozyme scaffold

Qiu, Shuai; Xu, Shen-Yuan; Wang, Ya-Jun; Zheng, Yu-Guo, Chemical Engineering Science, 2022, 261,

Production Method 13

638-07-3

90866-33-4

Reaction Conditions

1.1 Reagents: Glucose ,  NADH Catalysts: 2613440-02-9 Solvents: Water ;  840 min, pH 7, 30 °C

Reference

Preparation of combined cross-linked enzyme aggregates and its application in synthesis of chiral alcohols by the asymmetric reduction of carbanyl group

Yang, Meng; Jiang, Huijuan; Ning, Chenxi; Wei, Dongzhi; Su, Erzheng, Gaodeng Xuexiao Huaxue Xuebao, 2018, 39(1), 54-63

Production Method 14

638-07-3

90866-33-4

86728-85-0

Reaction Conditions

1.1 Solvents: Water ;  72 h, 28 °C
1.2 Reagents: Hydrochloric acid Solvents: Water ;  acidified

Reference

Diplogelasinospora grovesii IMI 171018, a new whole cell biocatalyst for the stereoselective reduction of ketones

Carballeira, Jose D.; et al, Tetrahedron: Asymmetry, 2004, 15(6), 951-962

(R)-Ethyl 4-Chloro-3-hydroxybutanoate (~90%) Raw materials

• ethyl 4-chloro-3-oxobutanoate
• (R)-(+)-4-Chloro-3-hydroxybutyronitrile

(R)-Ethyl 4-Chloro-3-hydroxybutanoate (~90%) Preparation Products

• Ethyl (S)-4-chloro-3-hydroxybutyrate (86728-85-0)
• (R)-Ethyl 4-Chloro-3-hydroxybutanoate (~90%) (90866-33-4)

• 1. An alkynylboronatecycloaddition strategy to functionalised benzyne derivatives?
  James D. Kirkham,Patrick M. Delaney,George J. Ellames,Eleanor C. Row,Joseph P. A. Harrity Chem. Commun., 2010,46, 5154-5156
• 2. An integrated chip for immunofluorescence and its application to analyze lysosomal storage disorders
  Jie Shen,Ying Zhou,Tu Lu,Junya Peng,Zhixiang Lin,Yuhong Pang,Li Yu Lab Chip, 2012,12, 317-324
• 3. Aggregation kinetics and gel formation in modestly concentrated suspensions of oppositely charged model ceramic colloids: a numerical study
  M. A. Piechowiak,A. Videcoq,R. Ferrando,D. Bochicchio,C. Pagnoux,F. Rossignol Phys. Chem. Chem. Phys., 2012,14, 1431-1439
• 4. An algal process treatment combined with the Fenton reaction for high concentrations of amoxicillin and cefradine
  Haitao Li,Yu Pan,Zhizhi Wang,Shan Chen,Ruixin Guo,Jianqiu Chen RSC Adv., 2015,5, 100775-100782
• 5. An all-solid-state imprinted polymer-based potentiometric sensor for determination of bisphenol S?
  Rongning Liang,Tanji Yin,Ruiqing Yao,Wei Qin RSC Adv., 2016,6, 73308-73312

• Solvents and Organic Chemicals Organic Compounds Organic acids and derivatives Hydroxy acids and derivatives Beta hydroxy acids and derivatives

• 828276-57-9(Butanoic acid, 4-chloro-3-hydroxy-, 1-methylpropyl ester)
• 10488-19-4(4-Chloro-3-Hydroxy Butanoic Ethyl Ester)
• 10488-69-4(Ethyl 4-chloro-3-hydroxybutanoate)
• 86728-99-6(Butanoic acid, 4-chloro-3-hydroxy-, octyl ester, (R)-)
• 89693-37-8(Butanoic acid, 4-chloro-3-hydroxy-, 1-methylethyl ester)
• 86728-85-0(Ethyl (S)-4-chloro-3-hydroxybutyrate)
• 828276-56-8(Butanoic acid, 4-chloro-3-hydroxy-, 2-methylpropyl ester)
• 100596-47-2(Butanoic acid, 4-chloro-3-hydroxy-, propyl ester)
• 185033-56-1(Butanoic acid, 4-chloro-3-hydroxy-, butyl ester)
• 88496-70-2((R)-4-Chloro-3-hydroxybutyric Acid Methyl Ester)