Detection of interaction between protein trytophan residues and small or macromolecular ligands by synchrotron radiation magnetic circular dichroism?

Analytical Methods Pub Date: 2014-12-11 DOI: 10.1039/C4AY02618G

Abstract

Presented herein is a novel method for the detection of tryptophan–ligand interactions utilising magnetic circular dichroism. Two examples are presented, one a small molecule and another, a large polysaccharide, heparan sulfate. These data are also the first reported example of a Trp–glycosaminoglycan interaction shown by magnetic circular dichroism.

Graphical abstract: Detection of interaction between protein trytophan residues and small or macromolecular ligands by synchrotron radiation magnetic circular dichroism
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