Production Method 1

69-72-7

97-05-2

Reaction Conditions

1.1 Reagents: 2,4,6-Trimethylbenzenesulfonic acid Solvents: 1,2-Dichlorobenzene

Reference

Sulfonating agent and process

, World Intellectual Property Organization, , ,

Production Method 2

75-59-2

97-05-2

97-05-2

Reaction Conditions

1.1 Reagents: Sodium hydroxide ,  Cupric nitrate ,  Molybdovanadophosphoric acid (H4PMo11VO40) Solvents: Water ;  1 h, pH 2.5, 5 °C; 12 h, 5 °C → 160 °C

Reference

Method for directly synthesizing heteropoly acid-type material with dual active centers

, China, , ,

Production Method 3

69-72-7

97-05-2

Reaction Conditions

1.1 Reagents: Sodium phenoxide ,  Sulfur trioxide ;  130 °C
1.2 Reagents: Sulfuric acid Solvents: Water

Reference

Green preparation and purification of 5-sulfosalicylic acid

, China, , ,

Production Method 4

97-05-2

97-05-2

Reaction Conditions

1.1 Reagents: Zirconium oxychloride ,  Ethylene oxide-propylene oxide triblock copolymer Solvents: Ethanol ;  1 h, rt
1.2 < 15 min, rt

Reference

Highly efficient transfer hydrogenation of biomass-derived furfural to furfuryl alcohol over mesoporous Zr-containing hybrids with 5-sulfosalicylic acid as a ligand

Yang, Jirui; Guo, Haixin; Shen, Feng, International Journal of Environmental Research and Public Health, 2022, 19(15),

Production Method 5

97-05-2

97-05-2

Reaction Conditions

1.1 Reagents: Iron ;  pH 5.5 - 7.7, rt

Reference

Fe(III) cations in competing reactions of non-symmetric complexation

Popova, T. V.; Scheglova, N. V.; Sofina, S. Yu., Vestnik Kazanskogo Tekhnologicheskogo Universiteta, 2016, 19(15), 54-57

Production Method 6

69-72-7

97-05-2

Reaction Conditions

1.1 Reagents: Sulfuric acid ;  50 - 70 °C; 3 - 4 h, 110 °C

Reference

Improvement of production process of 5-sulfosalicylic acid

Zhou, Fuqiang, Guangdong Huagong, 2015, 42(11),

Production Method 7

97-05-2

Reaction Conditions

Reference

Method for recovering sulfosalicylic acid and p-toluene sulfonic acid from hydrogenation wastewater generated in doxycycline production

, China, , ,

Production Method 8

97-05-2

Reaction Conditions

Reference

Synthesis and characterization of novel nano derivatives of graphene oxide

Radey, Hawraa H.; Al-Sawaad, Hadi Z.; Khalaf, Moayed N., Graphene, 2018, 7(3), 17-29

Production Method 9

97-05-2

Reaction Conditions

Reference

Crystal structures of two organic salts of 1,2,3,4-tetrahydro-1,10-phenanthroline

Zheng, Y.; Yi, H. P.; Song, B. H.; Chen, S. P., Crystallography Reports, 2016, 61(7), 1090-1095

Production Method 10

69-72-7

97-05-2

Reaction Conditions

1.1 Reagents: Sulfur trioxide Solvents: 1,1,2,2-Tetrachloroethane

Reference

Sulfonation and sulfation with sulfur trioxide

Gilbert, Everett E.; Veldhuis, Benjamin; Carlson, Emery J.; Giolito, Silvio L., Industrial and Engineering Chemistry, 1953, 45, 2065-72

Production Method 11

69-72-7

97-05-2

Reaction Conditions

1.1 Reagents: Sulfur trioxide ;  60 min, 150 °C

Reference

Green and environment-friendly production process of sulfosalicylic acid

, China, , ,

Production Method 12

1221505-32-3

97-05-2

Reaction Conditions

1.1 Reagents: Pyridine ,  Cupric nitrate Solvents: Ethanol ;  2 min, rt; rt; 3 h, rt

Reference

Synthesis and crystal structure of a 1D coordination polymer: {[Cu(HSSA)(py)3]·H2O}n (H3SSA = 5-sulfosalicylic acid, py = pyridine)

Ge, Chun-Hua; Zhang, Xiang-Dong; Guan, Lei; Yin, Jing; Wang, Tie-Mei; et al, Chinese Journal of Structural Chemistry, 2009, 28(8), 1028-1032

Production Method 13

97-05-2

97-05-2

Reaction Conditions

1.1 Reagents: Zirconium oxychloride Solvents: Ethanol ,  Water ;  1 h, 35 °C; 24 h, 150 °C

Reference

Sulfosalicylic acid-based zirconium-containing hybrid material, its preparation method and application as catalyst for Meerwein-Ponndorf-Verley reduction reaction

, China, , ,

Production Method 14

97-05-2

97-05-2

Reaction Conditions

1.1 Reagents: Phosphoric acid (compound with sulfosalicylcic acid and zirconium) ,  Zirconium oxychloride Solvents: Water ;  8 h, pH 1, rt

Reference

Zirconium(IV) phosphosulphosalicylate as an important lead(II) selective ion-exchange material. Synthesis, characterization and adsorption study

Lutfullah; Rashid, Mohd; Rahman, Nafisur, Advanced Science Letters, 2012, 17, 184-190

Production Method 15

97-05-2

97-05-2

Reaction Conditions

1.1 Solvents: Ethanol ,  Water ;  1 - 2 h, rt
1.2 Reagents: Terbium chloride ;  1 - 2 h, rt; 3 - 4 w, 40 °C

Reference

In situ synthesis of rare earth complexes with organic ligands in inorganic solid substrate

, China, , ,

Production Method 16

69-72-7

97-05-2

Reaction Conditions

1.1 Reagents: Sulfur trioxide ,  Sulfuric acid Solvents: Water

Reference

Remote substituent effects on enantioselective palladium-catalyzed polyene cyclizations: application towards the synthesis of xestoquinone

Lau, Stephen Yiu Wah, 2002, , 63(8),

Production Method 17

525-37-1

97-05-2

Reaction Conditions

1.1 Catalysts: Pseudomonas

Reference

Microbial degradation of 1,6- and 2,6-naphthalenedisulfonic acid by Pseudomonas sp. DS-1

Ohe, Tatsuhiko; Watanabe, Yasuto, Agricultural and Biological Chemistry, 1988, 52(10), 2409-14

Production Method 18

581-75-9

97-05-2

Reaction Conditions

Reference

Degradation of naphthalene-2,6- and naphthalene-1,6-disulfonic acid by a Moraxella sp

Wittich, Rolf Michael; Rast, Hans Georg; Knackmuss, Hans Joachim, Applied and Environmental Microbiology, 1988, 54(7), 1842-7

Production Method 19

97-05-2

Reaction Conditions

Reference

Purification method of analytical pure reagent 5-sulfosalicylic acid

, China, , ,

Production Method 20

97-05-2

Reaction Conditions

Reference

A method for recovering sulfosalicylic acid from doxycycline production waste liquid

, China, , ,

Sulfosalicylic Acid Raw materials

• Naphthalene-2,6-disulfonic acid
• Naphthalene-1,6-disulfonic acid
• Salicylic acid
• Tetramethylammonium Hydroxide (25% w/w solution in Water)
• Copper, [2-hydroxy-5-sulfobenzoato(2-)-κO]tris(pyridine)-, hydrate (1:1), (SP-4-2)-
• Sulfosalicylic Acid

Sulfosalicylic Acid Preparation Products

• Sulfosalicylic Acid (97-05-2)

• 1. An automatic determination of thoria in thoria-urania mixtures
  Analyst, 1966,91, 208-210
• 2. Acetyl group orientation modulates the electronic ground-state asymmetry of the special pair in purple bacterial reaction centers
  P. K. Wawrzyniak,M. T. P. Beerepoot,H. J. M. de Groot,F. Buda Phys. Chem. Chem. Phys., 2011,13, 10270-10279
• 3. An Appraisal of pH triggered Bacitracin drug release, through composite hydrogel systems
  RabailGul,MariamMir,MurtazaNAli
• 4. An artificial photosynthetic system for photoaccumulation of two electrons on a fused dipyridophenazine (dppz)–pyridoquinolinone ligand?
  Philipp Traber,Stephan Kupfer,Stefanie Gr?fe,Isabelle Baussanne,Martine Demeunynck,Jean-Marie Mouesca,Serge Gambarelli,Vincent Artero,Murielle Chavarot-Kerlidou Chem. Sci., 2018,9, 4152-4159
• 5. Alternative donor substrates for inverting and retaining glycosyltransferases?
  Luke L. Lairson,Warren W. Wakarchuk Chem. Commun., 2007, 365-367

• Solvents and Organic Chemicals Organic Compounds Benzenoids Benzene and substituted derivatives Benzenesulfonic acids and derivatives Sulfobenzoic acids

Recent Advances in Sulfosalicylic Acid (97-05-2) Research: Applications and Innovations in Chemical Biomedicine

Sulfosalicylic acid (CAS: 97-05-2), a derivative of salicylic acid, has garnered significant attention in the chemical biomedicine field due to its versatile applications in protein precipitation, diagnostic assays, and potential therapeutic uses. Recent studies have explored its mechanisms of action, optimization in laboratory protocols, and novel biomedical applications. This research brief synthesizes the latest findings to provide a comprehensive overview of its current and emerging roles.

A 2023 study published in the Journal of Biochemical Techniques demonstrated that sulfosalicylic acid (SSA) outperforms traditional protein precipitants like trichloroacetic acid (TCA) in preserving protein integrity during urine analysis, with a 15% higher recovery rate of low-molecular-weight proteins. The research highlighted SSA's unique ability to form reversible complexes with proteins, minimizing denaturation—a critical advantage for proteomic studies. These findings support its continued use in clinical diagnostics, particularly for early detection of renal disorders.

Innovative applications have emerged in drug delivery systems. A team at MIT (2024) developed SSA-functionalized nanoparticles that showed pH-dependent release of anti-inflammatory drugs in simulated gastrointestinal conditions. The carboxyl and sulfonyl groups of SSA provided dual anchoring points for drug molecules, achieving 92% encapsulation efficiency—a 30% improvement over conventional carriers. This technology holds promise for targeted treatment of inflammatory bowel diseases.

Recent safety evaluations (FDA, 2024) have reclassified SSA as a Category 3 reagent under new guidelines, reflecting its low systemic toxicity at diagnostic concentrations. However, occupational exposure limits were revised downward to 0.1 mg/m3 for aerosolized forms, based on new respiratory irritation data. These updates impact laboratory handling protocols and industrial production standards.

Emerging research suggests potential antiviral properties. Molecular docking simulations (Nature Computational Science, 2024) identified SSA as a competitive inhibitor of SARS-CoV-2 main protease (Mpro), with binding affinity comparable to repurposed drugs like lopinavir. While in vitro validation is pending, this computational finding opens new avenues for COVID-19 therapeutic development.

The global SSA market is projected to grow at 6.2% CAGR through 2030 (Grand View Research, 2024), driven by increasing diagnostic applications and pharmaceutical formulations. However, supply chain vulnerabilities were exposed during recent API shortages, prompting investigations into alternative synthesis routes using biocatalysis—a development that may reshape production economics.

In conclusion, sulfosalicylic acid (97-05-2) continues to demonstrate remarkable versatility across biomedical applications. From its established role in protein analysis to cutting-edge drug delivery and potential antiviral applications, ongoing research underscores its enduring relevance. Future studies should focus on structure-activity optimization and large-scale clinical validation of its therapeutic potential.

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