5-CFDA Chemical and Physical Properties

Names and Identifiers

- 3',6'-Diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid
- 5-Carboxyfluorescein diacetate
- 5(6)-CARBOXYFLUORESCEIN DIACETATE
- BIOCHEMIKA, FOR FLUORESCENCE, ≥95.0 (HPLC)
- Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylicacid, 3',6'-bis(acetyloxy)-3-oxo-
- 5-CFDA
- 5-Carboxy-di-O-acetylfluorescein
- 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
- Cfda-5
- BIC1071
- WPUZGNPQMIWOHE-UHFFFAOYSA-N
- EBD121
- 3′,6′-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid (ACI)
- AS-60165
- AKOS015902367
- C2859
- DTXSID00229971
- FT-0620191
- CHEMBL493815
- MFCD00036872
- SCHEMBL6663204
- 3',6'-bis(acetyloxy)-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
- 3',6'-Bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-5-carboxylic acid
- 5-Carboxyfluorescein Diacetate - CAS 79955-27-4
- 5-Carboxyfluorescein diacetate, ~95% (HPLC)
- CS-3525
- J-100071
- 79955-27-4
- A11047
- PD063611
- HY-D0047
- 5-Carboxyfluoresceindiacetate
- A864776
- Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-5-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo-
- DTXCID50152462
- DA-49814
- MDL： MFCD00467494
- Inchi： 1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
- InChI Key： WPUZGNPQMIWOHE-UHFFFAOYSA-N
- SMILES： O=C(C1C=C2C(OC3(C4C(=CC(=CC=4)OC(C)=O)OC4C3=CC=C(C=4)OC(C)=O)C2=CC=1)=O)O

Computed Properties

Exact Mass: 460.07900
Monoisotopic Mass: 460.07943208g/mol
Isotope Atom Count: 0
Hydrogen Bond Donor Count: 1
Hydrogen Bond Acceptor Count: 9
Heavy Atom Count: 34
Rotatable Bond Count: 5
Complexity: 828
Covalently-Bonded Unit Count: 1
Defined Atom Stereocenter Count: 0
Undefined Atom Stereocenter Count : 0
Defined Bond Stereocenter Count: 0
Undefined Bond Stereocenter Count: 0
Topological Polar Surface Area: 125
Surface Charge: 0
Tautomer Count: nothing
XLogP3: 3.2

Experimental Properties

Color/Form: White or nearly white crystals or powders
Melting Point: >250°C
Boiling Point: 693.1±55.0 °C at 760 mmHg
Flash Point: 241.9±25.0 °C
Solubility: DMSO: soluble
PSA: 125.43000
LogP: 3.80340
pka: 6.4(at 25℃)
λmax: <300nm
PH: Weak green ' uorescence (6.0) to strong green ' uourescence (7.2)
Solubility: Soluble in ethanol, acetone, dmso

5-CFDA Security Information

Symbol:
Prompt：warning
Signal Word：Danger
Hazard Statement： H301,H311,H315,H319,H331,H335,H350,H360
Warning Statement： P201,P261,P280,P301+P310,P305+P351+P338,P311
Hazardous Material transportation number：NONH for all modes of transport
WGK Germany：3
Hazard Category Code： R45;R46;R23/24/25;R36/37/38
Safety Instruction： 53-22-26-36/37/39-45
FLUKA BRAND F CODES：10-21
Hazardous Material Identification：
Storage Condition：-20°C, protect from light
Risk Phrases：R45; R46; R23/24/25; R36/37/38
Safety Term：53-22-26-36/37/39-45

5-CFDA Pricemore >>

Related Categories	No.	Product Name	Cas No.	Purity	Specification	Price	update time
SHANG HAI YI EN HUA XUE JI SHU Co., Ltd.	R024759-100mg	5-CFDA	79955-27-4	95%	100mg	￥572	2023-09-07
SHANG HAI YI EN HUA XUE JI SHU Co., Ltd.	R024759-25mg	5-CFDA	79955-27-4	95%	25mg	￥211	2023-09-07
SHANG HAI YI EN HUA XUE JI SHU Co., Ltd.	R024759-500mg	5-CFDA	79955-27-4	95%	500mg	￥1683	2023-09-07
TRC	C178345-50mg	5-Carboxyfluorescein Diacetate	79955-27-4		50mg	$ 104.00	2023-04-18
TRC	C178345-250mg	5-Carboxyfluorescein Diacetate	79955-27-4		250mg	$ 236.00	2023-04-18
TRC	C178345-500mg	5-Carboxyfluorescein Diacetate	79955-27-4		500mg	$ 437.00	2023-04-18
SHANG HAI XIAN DING Biotechnology Co., Ltd.	C2859-50mg	5-CFDA	79955-27-4	93.0%(LC&T)	50mg	￥485.0	2022-05-30
SHANG HAI XIAN DING Biotechnology Co., Ltd.	C2859-200mg	5-CFDA	79955-27-4	93.0%(LC&T)	200mg	￥1040.0	2022-05-30
WU HAN AN JIE KAI Biomedical Technology Co., Ltd.	ajce51220-50mg	5-CFDA (5-Carboxyfluorescein diacetate)	79955-27-4	98%	50mg	￥445.00	2023-09-07
BAI LING WEI Technology Co., Ltd.	128132-50MG	5-Carboxyfluorescein diacetate, 95%	79955-27-4	95%	50MG	￥ 437	2022-04-26

5-CFDA Production Method

Production Method 1

76823-03-5

79955-27-4

Reaction Conditions

1.1 Reagents: Pyridine Solvents: Dimethylformamide , Dichloromethane ; 4 h, rt

Reference

New Fluorescein Precursors for Live Bacteria Detection

Guilini, Celia; et al, Analytical Chemistry (Washington, 2015, 87(17), 8858-8866

Production Method 2

76823-03-5

79955-27-4

Reaction Conditions

1.1 Solvents: Acetic anhydride ; 8 h, 160 °C

Reference

A protein-coupled fluorescent probe for organelle-specific imaging of Na+

Taguchi, Ryo; et al, Sensors and Actuators, 2018, 265, 575-581

Production Method 3

3301-79-9

76823-03-5

79955-27-4

3348-03-6

Reaction Conditions

1.1 Solvents: Pyridine ; 30 min, 110 °C

Reference

Separating the isomers-Efficient synthesis of the N-hydroxysuccinimide esters of 5 and 6-carboxyfluorescein diacetate and 5 and 6-carboxyrhodamine B

Brunet, Aurelie; et al, Bioorganic & Medicinal Chemistry Letters, 2014, 24(14), 3186-3188

Production Method 4

76823-03-5

79955-27-4

Reaction Conditions

1.1 Reagents: Pyridine , 6-Carboxyfluorescein

Reference

Transition metal catalyzed cyclization and synthesis of triptolide analogs

Pan, Jiehui, 2006, , 68(7),

Production Method 5

72088-94-9

79955-27-4

3348-03-6

Reaction Conditions

1.1 Reagents: Pyridine ; 5 h, 80 °C

Reference

Elucidating target specificity of the taccalonolide covalent microtubule stabilizers employing a combinatorial chemical approach

Du, Lin ; et al, Nature Communications, 2020, 11(1),

Production Method 6

552-30-7

79955-27-4

3348-03-6

Reaction Conditions

1.1 Reagents: Methanesulfonic acid ; 24 h, 80 °C
2.1 Reagents: Pyridine ; 5 h, 80 °C

Reference

Elucidating target specificity of the taccalonolide covalent microtubule stabilizers employing a combinatorial chemical approach

Du, Lin ; et al, Nature Communications, 2020, 11(1),

5-CFDA Raw materials

5-CFDA Preparation Products

5-CFDA Suppliers

Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd.

Gold Member

Audited Supplier

(CAS:79955-27-4)5-Carboxyfluorescein diacetate

Order Number:G10030102

Stock Status:in Stock

Quantity:1mg;5mg;100mg;

Purity:98%

Pricing Information Last Updated:Tuesday, 12 August 2025 16:32

Price ($):inquiry

Email:[email protected]

Product Official Link

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Cas no 79955-27-4 (5-CFDA)

5-CFDA Chemical and Physical Properties

Names and Identifiers

Computed Properties

Experimental Properties

5-CFDA Security Information

5-CFDA Pricemore >>

5-CFDA Production Method

Production Method 1

Production Method 2

Production Method 3

Production Method 4

Production Method 5

Production Method 6

5-CFDA Raw materials

5-CFDA Preparation Products

5-CFDA Suppliers

5-CFDA Related Literature

Related Categories

79955-27-4 (5-CFDA) Related Products