Biocatalytic kinetic resolution of d,l-pantolactone by using a novel recombinant d-lactonase
RSC Advances Pub Date: 2020-12-24 DOI: 10.1039/D0RA09053K
Abstract
D-Pantolactone is a key chiral intermediate for the synthesis of D-pantothenic acid and its derivatives. Biocatalytic kinetic resolution of D,L-pantoyl lactone using D-lactonase is an efficient route to synthesize D-pantolactone. In this study, we report the expression of a novel D-lactonase TSDL in Escherichia coli host. The recombinant TSDL exhibited high hydrolysis activity and enantioselectivity toward D-pantolactone. The reaction conditions of the recombinant TSDL-catalyzed kinetic resolution of D,L-pantolactone was systematically investigated by whole cell biocatalysis. In addition, a preparative-scale reaction for bioproduction of D-pantoic acid was examined under optimized reaction conditions. This study presented an alternative enzymatic process for kinetic resolution of D,L-pantolactone.
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Journal Name：RSC Advances
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CAS no.: 89640-58-4
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