Novel β-galactosynthase–β-mannosynthase dual activity of β-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates?

RSC Advances Pub Date: 2016-05-18 DOI: 10.1039/C6RA08060J

Abstract

A novel β-galactosynthase–β-mannosynthase dual-activity of β-galactosidase from Aspergillus oryzae was uncovered, for the first time, using free monosaccharide substrates. The enzyme successfully converted galactose and mannose monomer sugars efficiently into Gal-β(1–6)-Gal and Man-β(1–6)-Man, respectively. The discovery could potentially revolutionize chemoenzymatic glycan and non-digestible oligosaccharide (NDO) syntheses.

Graphical abstract: Novel β-galactosynthase–β-mannosynthase dual activity of β-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates
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