Chronoampermetric detection of enzymatic glucose sensor based on doped polyindole/MWCNT composites modified onto screen-printed carbon electrode as portable sensing device for diabetes?

RSC Advances Pub Date: 2022-10-06 DOI: 10.1039/D2RA04947C

Abstract

Doped-polyindole (dPIn) mixed with multi-walled carbon nanotubes (MWCNTs) were coated on a screen-printed electrode to improve the electroactive surface area and current response of the chronoamperometric enzymatic glucose sensor. Glucose oxidase mixed with chitosan (CHI-GOx) was immobilized on the electrode. (3-Aminopropyl) triethoxysilane (APTES) was used as a linker between the CHI-GOx and the dPIn. The current response of the glucose sensor increased with increasing glucose concentration according to a power law relation. The sensitivity of the CHI-GOx/APTES/dPIn was 55.7 μA mM?1 cm?2 with an LOD (limit of detection) of 0.01 mM, where the detectable glucose concentration range was 0.01–50 mM. The sensitivity of the CHI-GOx/APTES/1.5%MWCNT-dPIn was 182.9 μA mM?1 cm?2 with an LOD of 0.01 mM, where the detectable glucose concentration range was 0.01–100 mM. The detectable concentration ranges of glucose well cover the glucose concentrations in urine and blood. The fabricated enzymatic glucose sensors showed high stability during a storage period of four weeks and high selectivity relative to other interferences. Moreover, the sensor was successfully demonstrated as a continuous or step-wise glucose monitoring device. The preparation method employed here was facile and suitable for large quantity production. The glucose sensor fabricated here, consisting of the three-electrode cell of SPCE, were simple to use for glucose detection. Thus, it is promising to use as a prototype for real glucose monitoring for diabetic patients in the future.

Graphical abstract: Chronoampermetric detection of enzymatic glucose sensor based on doped polyindole/MWCNT composites modified onto screen-printed carbon electrode as portable sensing device for diabetes
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