A gold-based immunochromatographic strip for the specific detection of tacrolimus in whole blood?

Journal of Materials Chemistry B Pub Date: 2023-05-02 DOI: 10.1039/D3TB00569K

Abstract

Tacrolimus is a macrolide immunosuppressant widely used in organ transplantation. Due to the narrow treatment window, therapeutic drug monitoring of the clinical application of tacrolimus is necessary. In this study, a carboxyl group introduced at the hydroxyl or carbon positions of tacrolimus was used to couple with carrier protein to synthesize complete antigens. After screening different immunogens and coating antigens, a highly-sensitive and specific monoclonal antibody (mAb) 4C5 was obtained, with a half inhibitory concentration (IC50) value of 0.26 ng mL?1 determined by indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). A colloidal gold-based immunochromatographic strip (CG-ICS) was established to monitor tacrolimus in human whole blood based on the mAb 4C5. Through visual observation, it was found that the visual limit of detection (vLOD) and cut-off value of qualitative detection were 1.0 and 20.0 ng mL?1, respectively. The calculated LOD (cLOD) value of quantitative detection was 0.16 ng mL?1, and the linear range was 0.48–7.57 ng mL?1. In addition, the results of the CG-ICS for analyzing real positive samples of human whole blood were basically consistent with those of LC-MS/MS. Therefore, the CG-ICS was suitable for rapid and accurate clinical monitoring of tacrolimus.

Graphical abstract: A gold-based immunochromatographic strip for the specific detection of tacrolimus in whole blood
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