Substrate access path-guided engineering of l-threonine aldolase for improving diastereoselectivity?
Chemical Communications Pub Date: 2022-06-24 DOI: 10.1039/D2CC02644A
Abstract
The L-threonine aldolase from Leishmania major was engineered to improve its diastereoselectivity by a CAST/ISM strategy, providing insights into the relationship between the physico-chemical properties of the substrate access path and diastereoselectivity. The steric hindrance, hydrophobic interaction and π–π interaction cooperated to improve the diastereoselectivity of the enzyme, with a diastereomeric excess (de) value reaching 96.3%syn from 26.8%syn.
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Journal Name：Chemical Communications
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CAS no.: 89640-58-4
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