Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway?

Chemical Communications Pub Date: 2022-09-28 DOI: 10.1039/D2CC01799G

Abstract

Cyanobactins are linear and cyclic post-translationally modified peptides. Here we show that the prenyl-D-Arg-containing autumnalamide A is a member of the cyanobactin family. Biochemical assays demonstrate that the AutF prenyltransferase targets the guanidinium moiety in arginine and homoarginine and is a useful tool for biotechnological applications.

Graphical abstract: Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway
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