Deep eutectic solvents – a new additive in the encapsulation of lipase B from Candida antarctica: biocatalytic applications?

Reaction Chemistry & Engineering Pub Date: 2021-11-23 DOI: 10.1039/D1RE00469G

Abstract

Enzyme encapsulation in a sol–gel matrix can be improved using some additives acting on enzyme activity and/or stability. Ionic liquids, polyhydroxy compounds, sugars, etc. have been previously reported as additives. This study focuses on the possibility to use deep eutectic solvents as additives in the immobilization of lipase B from Candida antarctica (CaL-B) through sol–gel encapsulation. The transesterification of 1-phenylethanol was chosen as a model reaction. Process optimization was successfully performed regarding the most important reaction parameters (an alcohol/lipase weight ratio of 20?:?1, an alcohol/acyl donor molar ratio of 1?:?2, n-hexane as a solvent, 30 °C reaction temperature, vinyl acetate as an acyl donor and a substrate concentration of 100 mM). Under the optimal conditions, the reaction mediated by the most active biocatalyst (SG-3, containing a deep eutectic solvent formed from choline chloride and fructose, as a 30% aqueous solution) reached ～48% after only four hours, maintaining almost 85% of its initial activity even after eight reaction cycles.

Graphical abstract: Deep eutectic solvents – a new additive in the encapsulation of lipase B from Candida antarctica: biocatalytic applications
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