An enzyme-linked immunosorbent assay for the determination of tribenuron-methyl in water and soil using a molecularly imprinted film as an artificial antibody

Analytical Methods Pub Date: 2013-08-12 DOI: 10.1039/C3AY40880A

Abstract

This paper describes the development of a direct competitive enzyme-linked immunosorbent assay with a molecularly imprinted film as an artificial antibody to measure trace tribenuron-methyl (TBM) in environmental water and soil samples. The molecularly imprinted film was directly synthesized on the well surface of a MaxiSorp polystyrene 96-well plate by a non-covalent approach, and characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, and static and kinetic adsorption experiments. Under optimum conditions, the IC50 and IC15 of the established method are 19.73 μg L?1 and 0.30 μg L?1, respectively, and its cross-reactivity with three sulfonylurea herbicides is in the range of 3.17–13.10%. The method was successfully applied to the determination of TBM in spiked environmental water and soil samples with recoveries from 93.6 to 96.8% and 87.5 to 97.5%, respectively. The results are in good agreement with those obtained by high performance liquid chromatography.

Graphical abstract: An enzyme-linked immunosorbent assay for the determination of tribenuron-methyl in water and soil using a molecularly imprinted film as an artificial antibody
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