Synthesis of α-glycosidase hybrid nano-flowers and their application for enriching and screening α-glycosidase inhibitors
New Journal of Chemistry Pub Date: 2017-11-24 DOI: 10.1039/C7NJ03545D
Abstract
To rapidly and accurately screen compounds present in traditional Chinese herbal medicines for α-glycosidase inhibitors, we synthesized a novel nano-affinity material to enable the targeted screening and enrichment of α-glycosidase inhibitors. This nano-affinity material was synthesized by self-assembly involving α-glycosidase and Cu2+. A systematic analysis revealed the flower-like structure of this nano-material, with a diameter of 7.564 μm. The highest enzyme activity was 214.03% compared to free enzymes. This novel material was used to screen and enrich α-glycosidase inhibitors from Chinese rhubarb. Ten compounds were detected. Of these, L-epicatechin and anthraquinone displayed inhibitory activity. This novel nano-hybrid affinity material allows the rapid screening and separation of active components in complex systems.
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Journal Name：New Journal of Chemistry
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CAS no.: 89640-58-4
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