Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes?

Chemical Communications Pub Date: 2010-11-15 DOI: 10.1039/C0CC02924F

Abstract

A minimal enriched P450 BM3 library was screened for the ability to oxidize inert cyclic and acyclic alkanes. The F87A/A328V mutant was found to effectively hydroxylate cyclooctane, cyclodecane and cyclododecane. F87V/A328F with high activity towards cyclooctane hydroxylated acyclic n-octane to 2-(R)-octanol (46% ee) with high regioselectivity (92%).

Graphical abstract: Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes
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