A validated liquid chromatography mass spectrometry method for the quantification of tinoridine hydrochloride in rat plasma and its application to pharmacokinetic studies

Analytical Methods Pub Date: 2015-01-07 DOI: 10.1039/C4AY02898H

Abstract

A selective, sensitive and fast liquid chromatography coupled with mass spectrometry method (LC-MS) has been developed and validated for the quantification of tinoridine hydrochloride in rat plasma using ketorolac tromethamine as an internal standard. Pre-treatment of the sample obtained from the plasma involved a simple and reliable protein precipitation method using methanol. A successful chromatographic separation was achieved on a Zorbax extended C18 column (50 mm × 2.1 mm, 1.8 μm) using acetonitrile and 0.1% formic acid as the mobile phase in gradient elution mode with a flow rate of 0.5 mL min?1. Detection was performed with positive ion electrospray ionization mass spectrometry using target ions of [M + H]+ at m/z 317 for tinoridine and m/z 256 for ketorolac in selective ion mode. The method was validated in the calibration range of 2–200 ng mL?1. All the validation parameters were well within the limits. This method was further successfully applied in pharmacokinetic studies of the drug in rats.

Graphical abstract: A validated liquid chromatography mass spectrometry method for the quantification of tinoridine hydrochloride in rat plasma and its application to pharmacokinetic studies
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