Simultaneous determination of three neonicotinoid insecticide residues and their metabolite in cucumbers and soil by QuEChERS clean up and liquid chromatography with diode-array detection?

Analytical Methods Pub Date: 2016-05-12 DOI: 10.1039/C6AY01161F

Abstract

A new sensitive, selective and validated HPLC-DAD method was developed for the simultaneous determination of three neonicotinoid insecticides named dinotefuran, thiamethoxam and Clothianidin with their metabolite 1-methyl-3-nitroguanidine in cucumbers and soil based on QuEChERS as a pretreatment procedure. In the QuEChERS procedure, cucumber samples were extracted with acetonitrile and cleaned using C18, while soil samples were extracted with a mixture of acetonitrile?:?dichloromethane (1?:?2). The HPLC conditions were optimized where the neonicotinoids were separated using a gradient elution of water?:?acetonitrile and a Synergy Hydro RP C18 column. Matrix matched calibration standards were prepared in cucumbers and soil to eliminate the interference of the matrix. Relative standard deviations (RSDs) were less than 2.08% for all of the recovery tests. The limits of detection (LODs) and quantification (LOQs) for the neonicotinoids were in the ranges of 0.01–0.08 μg g?1 and 0.03–0.24 μg g?1, respectively. The method was applied successfully to determine residues and rates of disappearance of the three neonicotinoids from cucumbers and soil and their half lives.

Graphical abstract: Simultaneous determination of three neonicotinoid insecticide residues and their metabolite in cucumbers and soil by QuEChERS clean up and liquid chromatography with diode-array detection
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