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Rationally-designed fluorescent lysine riboswitch probes?

Organic & Biomolecular Chemistry Pub Date: 2012-09-04 DOI: 10.1039/C2OB26160J

Abstract

Two fluorescent lysine amide analogs, in which the carboxyl end of lysine was covalently attached to dansyl or NBD groups through an ethylene glycol-based linker, were rationally designed and synthesized. Both probes showed high binding affinity to the lysine riboswitch in vitro and their fluorescence intensities decreased by riboswitch binding.

Graphical abstract: Rationally-designed fluorescent lysine riboswitch probes

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Organic & Biomolecular Chemistry

Journal Name：Organic & Biomolecular Chemistry

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