Simultaneous determination and pharmacokinetic study of polygalaxanthone III, tenuifolin, tenuifoliside A and tenuifoliside C in rat plasma by LC-MS/MS after oral administration?

Analytical Methods Pub Date: 2014-06-05 DOI: 10.1039/C4AY00449C

Abstract

For the first time, a rapid and specific LC-MS/MS method has been developed for the simultaneous analysis of polygalaxanthone III, tenuifolin, tenuifoliside A and tenuifoliside C in rat plasma, and was applied to the pharmacokinetics (PK) studies of those three compounds. The analysis was carried out on an Agilent Eclipse plus C18 reversed-phase column (100 × 4.6 mm, 3.5 μm) by gradient elution with methanol and ammonia (0.01%, v/v). The flow rate was 0.4 mL min?1. All analytes including internal standards (I.S.) were monitored by selected reaction monitoring with an electrospray ionization source. Linear responses were obtained for polygalaxanthone III and tenuifoliside A ranging from 1 to 2000 ng mL?1, and tenuifolin and tenuifoliside C ranging from 2.5 to 2000 ng mL?1. The intra-day and inter-day precisions (RSD) were less than 12.98% and 7.50% respectively. The extraction recovery ranged from 70.06 ± 6.33% to 86.95 ± 5.35%, and I.S. was 77.23 ± 3.68%. Stability studies showed that the accuracies of polygalaxanthone III, tenuifolin, tenuifoliside A and tenuifoliside C ranged from 93.18% to 106.70%. The matrix effects ranged from 91.72 ± 4.80 to 101.84 ± 5.04. The validated method was successfully used to determine the concentration–time profiles of polygalaxanthone III, tenuifolin, tenuifoliside A and tenuifoliside C.

Graphical abstract: Simultaneous determination and pharmacokinetic study of polygalaxanthone III, tenuifolin, tenuifoliside A and tenuifoliside C in rat plasma by LC-MS/MS after oral administration
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