Ratios of Fourier to orthogonal function coefficients: spectrophotometric determination of tetraenes in amphotericin B
ANALYSTANALYST Pub Date: DOI: 10.1039/AN9881300563
Abstract
Ratios of Fourier function coefficients to their analogous orthogonal function coefficients are proposed as purity indices during spectrophotometric analysis. Hence, a1/p2(where a1= coefficient of cosx and p2 is the coefficient of P2) calculated for a set of absorbances is independent of concentration and is sensitive to the presence of interferents. The concentration of tetraenes in amphotericin B has been determined by this ratio, calculated from absorbances measured at 6-nm intervals from 264 to 294 nm.
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Journal Name：ANALYSTANALYST
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CAS no.: 89640-58-4
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