New theophylline-activated Diels–Alderase ribozymes by molecular engineering

Organic & Biomolecular Chemistry Pub Date: 2008-11-20 DOI: 10.1039/B816726E

Abstract

Allosteric ribozymes were developed by rational design and step-wise optimization. These ribozymes accelerate a Diels–Alder reaction between anthracene and maleimide derivatives. The optimized sequence is efficiently activated by theophylline under single and multiple turnover conditions and distinguishes between structurally similar effector molecules.

Graphical abstract: New theophylline-activated Diels–Alderase ribozymes by molecular engineering
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