Fluorescence quenching and spectrophotometric methods for the determination of 6-mercaptopurine based on carbon dots

RSC Advances Pub Date: 2016-05-18 DOI: 10.1039/C6RA07675K

Abstract

It is of great significance to develop an eco-friendly and sensitive platform for the detection of 6-mercaptopurine (6-MP) because of its side effects and variable activity. Herein, fluorescence quenching and spectrophotometric methods for the detection of 6-mercaptopurine (6-MP) using carbon dots (CDs) as a fluorescence probe are established. 6-MP not only serves to shelter the CDs effectively from being quenched, but also to reverse the quenching and restore the fluorescence due to its ability to remove Hg2+ from the surface of CDs. After the experimental conditions are optimized, the linear range for the detection of 6-MP is 0.04 to 12 μmol L?1, and the detection limit is 0.01 μmol L?1. When it refers to the spectrophotometric method, the linear range is 0.08 to 12 μmol L?1 and the detection limit is 0.02 μmol L?1. The CDs with 6-MP are systematically characterized with transmission electron microscopy (TEM), ultraviolet-visible (UV-vis) absorption and so on. Furthermore, the proposed methods are not only expected to become a potential tool for the fast response of 6-MP but also possess the potential for practical application to be applied in the determination of 6-MP in human serum samples with satisfactory results.

Graphical abstract: Fluorescence quenching and spectrophotometric methods for the determination of 6-mercaptopurine based on carbon dots
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