A highly sensitive SERS probe for bisphenol A detection based on functionalized Au@Ag nanoparticles?

Analytical Methods Pub Date: 2018-10-24 DOI: 10.1039/C8AY01966E

Abstract

Bisphenol A (BPA) usually exists in daily plastic products and its harmful effect on human health has been a popular topic. However, many conventional analytical methods need long sample preparation and measurement time, and some BPA biosensors suffer from the limitation of complex probe modification. Herein, we designed a simple SERS-coded aptamer sensor for BPA detection based on the electrostatic interaction between chitosan encapsulated 4-MBA coated Au@Ag NPs and anti-BPA aptamers. The negatively charged nucleic acid anti-BPA aptamer will cause the aggregation of the positively charged noble metal nano-reinforced substrate in acid buffer systems, resulting in a significant enhancement of the Raman signal. The specific binding of BPA with nucleic acid anti-BPA aptamers reduced the degree of aggregation of nanoparticles and weakened the SERS signal. This SERS-coded aptamer sensor had a wide linear range of 0.01–1 ng mL?1 and the limit of detection (LOD) was as low as 2.8 pg mL?1, and the method was successfully applied to detect BPA in bottled water within 15 min. As a rapid, simple, ultrasensitive and highly selective analytical technique for the detection of BPA, this SERS-based method would be important in biomedical diagnostics, environmental monitoring and food safety.

Graphical abstract: A highly sensitive SERS probe for bisphenol A detection based on functionalized Au@Ag nanoparticles
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