Determination of the partition coefficient of isoquinoline alkaloids from Chelidonium majus by reversed phase thin layer chromatography?

New Journal of Chemistry Pub Date: 2020-04-08 DOI: 10.1039/D0NJ00307G

Abstract

Lipophilicity plays a significant role as a determinant of biological properties. Reversed-phase high-performance thin layer chromatography (HP-TLC) was applied to determine the log?P values of chelidonine, allocryptopine, sanguinarine chloride and chelerythrine chloride at three different pH values: 3.0, 7.4 and 10.0. The mobile phase was a mixture of acetonitrile and water with different concentrations of acetonitrile (50–75 vol%). The retention factors, Rm0 values, for the compounds with known log?P and for the analyzed substances were determined. The log?P values obtained experimentally were compared with the theoretical data obtained by using known algorithms (miLogP, xLogP, and AlogPs). Additionally, log?D values of the examined substances were determined. The highest log?P value was observed for chelerythrine chloride in an alkaline environment, while the lowest one was noted for chelidonine at acidic pH.

Graphical abstract: Determination of the partition coefficient of isoquinoline alkaloids from Chelidonium majus by reversed phase thin layer chromatography
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