Determination of iodine species in seaweed and seawater samples using ion-pair reversed phase high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry?

Analytical Methods Pub Date: 2012-08-20 DOI: 10.1039/C2AY25871D

Abstract

Ion-pair reversed phase high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (RP-HPLC-ICP-MS) has been used to determine two inorganic iodine species (I? and IO3?) and two organic iodinated amino acids (MIT and DIT) in seaweed and seawater samples within 5 min. The mobile phase used for a reversed phase C18 column was as follows: 2.0 mM tetraethylammonium hydroxide, 10 mM L-phenylalanine and 1% methanol (pH 8.9). Values of 1.5 mL min?1 for the flow rate of the mobile phase and 25 °C for the column temperature were selected for rapid separation of iodine species. The detection limits for IO3?, I?, MIT and DIT were 0.061, 0.052, 0.079 and 0.24 μg L?1, each, and the repeatabilities of peak height and peak area (20 μg L?1 for iodide and iodate, 40 μg L?1 for MIT, 100 μg L?1 for DIT) were all lower than 4%. Under the optimized conditions, the proposed method was successfully applied for the speciation analysis of iodine in seaweed and seawater samples. Recoveries of IO3?, I?, MIT and DIT in seaweed and seawater samples were between 90 and 110%. All results prove its advantages including high sensitivity, environmentally friendly, low time consumption, which are beneficial for routine analysis of iodine speciation in environmental and food fields.

Graphical abstract: Determination of iodine species in seaweed and seawater samples using ion-pair reversed phase high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry
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