Development of a monoclonal antibody-based immunochromatographic strip for the rapid detection of tigecycline in human serum
Analytical MethodsANAL METHODS-UK Pub Date: 2021-01-04 DOI: 10.1039/D0AY02182B
Abstract
In this study, a gold labelled immunochromatographic assay was developed to detect tigecycline (TGC) in human serum. For this purpose, an anti-TGC monoclonal antibody, 2G7, was produced and characterized, and was found to have a 50%-inhibitory concentration (IC50) of 2.303 ng mL?1 and a limit of detection (LOD) of 0.215 ng mL?1 for TGC. This strip assay had a visual limit of detection (vLOD) of 50 ng mL?1 and a cut-off value of 1000 ng mL?1 for TGC in human serum, when assessed by eye. With the aid of a strip scan reader, a quantitative determination of TGC was obtained with a calculated limit of detection (cLOD) of 15.03 ng mL?1. Analysis of TGC in human serum indicated that the results of our strip assay compared well with results obtained using ic-ELISA and LC-MS/MS. Therefore, this strip assay represents a sensitive and reliable method for the on-site detection of TGC in serum samples.
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Journal Name：Analytical MethodsANAL METHODS-UK
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CAS no.: 89640-58-4
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