• 1. Enabling shape memory and healable effects in a conjugated polymer by incorporating siloxane via dynamic imine bond?
  Yaling Zhang,Chunhui Dai,Shiwei Zhou,Bin Liu Chem. Commun., 2018,54, 10092-10095
• 2. Elusive 2-aminofuran Diels–Alder substrates for a straightforward synthesis of polysubstituted anilines?
  Ana G. Neo,Ana Bornadiego,Jesús Díaz,Stefano Marcaccini,Carlos F. Marcos Org. Biomol. Chem., 2013,11, 6546-6555
• 3. Evidence for the intrinsic nature of band-gap states electrochemically observed on atomically flat TiO2(110) surfaces?
  Shintaro Takata,Yoshihiro Miura Phys. Chem. Chem. Phys., 2014,16, 24784-24789
• 4. Establishing plasmon contribution to chemical reactions: alkoxyamines as a thermal probe?
  Olga Guselnikova,Gérard Audran,Jean-Patrick Joly,Andrii Trelin,Evgeny V. Tretyakov,Vaclav Svorcik,Oleksiy Lyutakov,Sylvain R. A. Marque Chem. Sci., 2021,12, 4154-4161
• 5. Enabling high-throughput single-animal gene-expression studies with molecular and micro-scale technologies
  Jason Wan Lab Chip, 2020,20, 4528-4538

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Azido-PEG₈-Amine (CAS No. 857891-82-8): A Versatile Platform for Advanced Chemical and Biomedical Applications

The compound Azido-PEG₈-Amine, identified by the CAS registry number 857891-82-8, represents a critical advancement in the field of bioconjugate chemistry. This molecule combines the unique properties of an azide group with a polyethylene glycol (PEG) spacer chain terminated by an amine functional group. The azidopropionyl PEG amine structure enables precise molecular engineering, making it indispensable in applications ranging from drug delivery systems to biomolecule labeling. Its modular design allows researchers to tailor reactivity, solubility, and biocompatibility, aligning with contemporary demands for highly specific biomedical tools.

The synthesis of Azido-PEG₈-Amine relies on well-established protocols in polymer chemistry, ensuring high purity and reproducibility. The eight-unit PEG spacer (molecular weight ~440 g/mol) balances hydrophilicity and flexibility, minimizing immunogenicity while facilitating efficient conjugation reactions. Recent studies highlight its compatibility with copper-free click chemistry (SPAAC reactions) under physiological conditions, a breakthrough for in vivo applications. For instance, a 2023 study published in Bioconjugate Chemistry demonstrated its use in stabilizing antibody-drug conjugates (ADCs) through site-specific attachment to cysteine residues, enhancing therapeutic efficacy while reducing off-target effects.

In the realm of diagnostics, the azide moiety of this compound serves as a pivotal component in bioorthogonal labeling strategies. When paired with strained cyclooctynes or tetrazines via rapid "snap-click" reactions, it enables real-time imaging of cellular processes without disrupting native biochemistry. A notable application involves tracking exosome-mediated intercellular communication—researchers at MIT recently utilized Azido-PEG₈-Amine-modified probes to visualize tumor-derived exosomes in live mice models, offering new insights into metastasis mechanisms.

The biomedical community has also leveraged this compound's amine terminus for protein functionalization. By conjugating to antibodies or enzymes via EDC/NHS coupling, scientists create bioactive constructs with extended circulation half-lives due to PEGylation. A 2024 review in Nature Protocols emphasized its role in engineering enzyme replacement therapies for lysosomal storage disorders—when linked to glucocerebrosidase, the PEG spacer reduced clearance by neonatal Fc receptors while preserving enzymatic activity.

Emerging applications extend into nanotechnology and materials science. Researchers at Stanford have integrated Azido-PEG₈-Amine into stimuli-responsive hydrogels where azide groups participate in redox-sensitive crosslinking networks. These systems release payloads only under disease-specific conditions like elevated glutathione levels in cancer cells, exemplifying smart drug delivery paradigms.

Pioneering work at the University of Tokyo further highlights its utility in DNA-encoded libraries (DEL). The compound's bifunctionality allows simultaneous attachment to DNA oligonucleotides and small molecule ligands via sequential click reactions—a method that increased library diversity by 40% while maintaining structural integrity during high-throughput screening.

Safety profiles validated through recent toxicology studies affirm its biocompatibility at therapeutic concentrations. In vitro cytotoxicity assays using HEK293 and primary hepatocyte cultures showed no significant adverse effects up to 1 mM concentrations—a critical threshold for clinical translation—while pharmacokinetic data from rodent models indicate rapid renal clearance of unconjugated material within 24 hours.

This multifunctional reagent continues to drive innovation across disciplines due to its modular architecture and proven performance under physiological constraints. As highlighted by a 2025 perspective article in Accounts of Chemical Research, its ability to bridge organic synthesis with biological systems positions it as a cornerstone material for next-generation theranostics—integrated diagnostic and therapeutic platforms that personalize patient care through precise molecular interactions.

• 516493-93-9(Azido-PEG5-amine)
• 182760-73-2(1,14-Diazido-3,6,9,12-tetraoxatetradecane)
• 356046-26-9(Azido-PEG5-azide)
• 166388-57-4(Azido-PEG2-C2-amine)
• 352439-36-2(Azide-PEG8-alcohol)
• 86770-67-4(Azido-PEG4-alcohol)
• 134179-38-7(Amino-PEG3-C2-Azido)
• 101187-39-7(Azido-PEG3-azide)
• 86520-52-7(Azido-PEG3-alcohol)
• 89485-61-0(m-PEG12-azide)