Cas no 80008-69-1 (5-Bromo-3-indolyl phosphate p-toluidine salt)

5-Bromo-3-indolyl phosphate p-toluidine salt is a chromogenic substrate widely used in alkaline phosphatase (AP) assays, particularly in immunohistochemistry and Western blotting. Upon enzymatic hydrolysis by AP, it yields an insoluble indigo-blue precipitate, facilitating clear visualization of target proteins or nucleic acids. Its high sensitivity and low background interference make it suitable for applications requiring precise localization. The p-toluidine salt form enhances solubility in aqueous buffers, ensuring consistent performance. This substrate is valued for its stability, reproducibility, and compatibility with various detection systems. It is commonly employed in research and diagnostic settings where robust and reliable signal amplification is critical.
5-Bromo-3-indolyl phosphate p-toluidine salt structure
80008-69-1 structure
Product Name:5-Bromo-3-indolyl phosphate p-toluidine salt
CAS No:80008-69-1
MF:C15H16BrN2O4P
MW:399.176303863525
CID:720072
PubChem ID:91658930
Update Time:2025-11-02

5-Bromo-3-indolyl phosphate p-toluidine salt Chemical and Physical Properties

Names and Identifiers

    • 5-Bromo-3-indolyl- phosphate p-toluidine salt
    • (5-bromo-1H-indol-3-yl) phosphate,(4-methylphenyl)azanium
    • 5-BROMO-3-INDOXYL PHOSPHATE, P-TOLUIDINE SALT
    • S07-0043
    • S07-043
    • W0421
    • 4-Methyl-benzenamine compd. with 5-bromo-1H-indol-3-yl dihydrogen phosphate
    • 5-Bromo-3-indolyl phosphate p-toluidine salt
    • 5-Bromo-1H-indol-3-ol dihydrogen phosphate (ester) compd with 4-methylbenzenamine
    • Blue phosphate, p-toluidine salt
    • 5-bromo-1H-indol-3-yl dihydrogen phosphate
    • (5-bromo-1H-indol-3-yl) phosphate
    • 5-Bromo-3-Indoxyl Phosphate p-Toluidine Salt extrapure, 98%
    • 80008-69-1
    • CS-0179170
    • 5-Bromo-3-indolyl phosphate, p-toluidine salt
    • MFCD03427361
    • 5-BROMO-3-INDOLYLPHOSPHATEP-TOLUIDINESALT
    • (5-bromo-1H-indol-3-yl) dihydrogen phosphate;4-methylaniline
    • Inchi: 1S/C8H7BrNO4P.C7H9N/c9-5-1-2-7-6(3-5)8(4-10-7)14-15(11,12)13;1-6-2-4-7(8)5-3-6/h1-4,10H,(H2,11,12,13);2-5H,8H2,1H3
    • InChI Key: MTKYSMVJOACNBO-UHFFFAOYSA-N
    • SMILES: CC1C=CC(N)=CC=1.O(C1=CNC2C=CC(Br)=CC1=2)P(O)(O)=O

Computed Properties

  • Exact Mass: 398.00300
  • Monoisotopic Mass: 398.00311g/mol
  • Isotope Atom Count: 0
  • Hydrogen Bond Donor Count: 4
  • Hydrogen Bond Acceptor Count: 5
  • Heavy Atom Count: 23
  • Rotatable Bond Count: 2
  • Complexity: 347
  • Covalently-Bonded Unit Count: 2
  • Defined Atom Stereocenter Count: 0
  • Undefined Atom Stereocenter Count : 0
  • Defined Bond Stereocenter Count: 0
  • Undefined Bond Stereocenter Count: 0
  • Topological Polar Surface Area: 109?2

Experimental Properties

  • PSA: 118.38000
  • LogP: 4.56030

5-Bromo-3-indolyl phosphate p-toluidine salt Security Information

5-Bromo-3-indolyl phosphate p-toluidine salt Customs Data

  • HS CODE:2933998090

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Additional information on 5-Bromo-3-indolyl phosphate p-toluidine salt

5-Bromo-3-indolyl phosphate p-toluidine salt (CAS 80008-69-1): A Comprehensive Guide

The 5-Bromo-3-indolyl phosphate p-toluidine salt (CAS 80008-69-1) is a specialized chemical compound widely recognized in biochemical and diagnostic applications. This compound, often referred to as BCIP p-toluidine salt, serves as a crucial substrate in enzymatic reactions, particularly in alkaline phosphatase assays. Its unique properties make it indispensable in molecular biology, immunology, and histochemistry.

One of the most notable features of 5-Bromo-3-indolyl phosphate p-toluidine salt is its role in colorimetric detection. When hydrolyzed by alkaline phosphatase, it produces an insoluble blue indigo dye, which is highly visible under microscopic examination. This characteristic has made it a preferred choice for Western blotting, ELISA, and in situ hybridization techniques. Researchers frequently inquire about its solubility, stability, and optimal storage conditions, which are critical for maintaining its efficacy in laboratory settings.

The compound's molecular formula, C15H16BrN2O4P, reflects its intricate structure, combining a brominated indole moiety with a phosphate ester. This design enhances its reactivity and specificity in enzymatic assays. Recent advancements in biomedical research have spurred interest in 5-Bromo-3-indolyl phosphate p-toluidine salt, particularly in studies involving cell signaling pathways and protein expression analysis. Its compatibility with nitroblue tetrazolium (NBT) further amplifies its utility in dual-substrate systems.

From a commercial perspective, the demand for BCIP p-toluidine salt has grown steadily, driven by its applications in diagnostic kits and research reagents. Suppliers often highlight its high purity (>98%) and low background noise, which are essential for sensitive assays. Questions about its synthesis, alternative substrates, and cost-effectiveness are common among procurement specialists, reflecting the compound's significance in budget-conscious laboratories.

In the context of SEO optimization, this article addresses frequently searched queries such as "What is the role of 5-Bromo-3-indolyl phosphate in Western blotting?" and "How does BCIP p-toluidine salt compare to other phosphatase substrates?" By integrating these long-tail keywords, we enhance the article's visibility while providing actionable insights for researchers and industry professionals.

Looking ahead, the versatility of 5-Bromo-3-indolyl phosphate p-toluidine salt ensures its continued relevance in emerging fields like single-cell analysis and high-throughput screening. Its ability to deliver reproducible results under varying experimental conditions makes it a staple in modern laboratories. For those seeking detailed protocols or troubleshooting tips, this compound's technical documentation and safety data sheets remain invaluable resources.

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