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• Solvents and Organic Chemicals Organic Compounds Organic acids and derivatives Organic phosphoric acids and derivatives Phosphate esters Aryl phosphates

Comprehensive Guide to 6-Chloro-3-indolyl phosphate p-toluidine salt (CAS No. 159954-33-3): Properties, Applications, and Research Insights

6-Chloro-3-indolyl phosphate p-toluidine salt (CAS No. 159954-33-3) is a specialized biochemical reagent widely recognized for its role in enzymatic assays, particularly in alkaline phosphatase-based detection systems. This compound, often abbreviated as BCIP p-toluidine salt, serves as a chromogenic substrate that yields an insoluble indigo-blue precipitate upon enzymatic hydrolysis. Its unique properties make it indispensable in molecular biology, immunohistochemistry, and diagnostic applications, aligning with current trends in precision medicine and high-throughput screening technologies.

The growing demand for sensitive detection methods in life sciences has propelled the relevance of 6-Chloro-3-indolyl phosphate p-toluidine salt. Researchers frequently search for alternatives to fluorescent probes due to photobleaching concerns, making this compound a preferred choice for colorimetric assays. Its stability under physiological pH ranges and compatibility with NBT (Nitro Blue Tetrazolium) for dual-chromogen systems further enhance its utility. Recent studies highlight its application in CRISPR-Cas9 validation assays and spatial transcriptomics, addressing modern scientific inquiries about gene editing accuracy and tissue-specific gene expression profiling.

From a structural perspective, the p-toluidine salt moiety of CAS 159954-33-3 improves solubility in aqueous buffers while maintaining substrate specificity for phosphatases. This characteristic is critical for ELISA and Western blotting protocols, where consistent signal generation is paramount. Industry professionals often inquire about optimizing signal-to-noise ratios in blotting experiments—a challenge where this compound excels due to its low endogenous interference in biological samples. Furthermore, its adoption in microarray technologies and lateral flow assays demonstrates versatility across diagnostic platforms.

Emerging applications of 6-Chloro-3-indolyl phosphate p-toluidine salt extend to 3D bioprinting quality control, where enzymatic markers verify cell viability in printed constructs. This aligns with the booming interest in regenerative medicine and organ-on-chip systems. Environmental scientists also utilize this substrate to monitor alkaline phosphatase activity in soil and water ecosystems, reflecting cross-disciplinary relevance. Notably, its non-radioactive nature addresses safety concerns prevalent in laboratory settings, making it compliant with green chemistry initiatives.

Quality considerations for CAS 159954-33-3 emphasize HPLC-verified purity (>98%) and batch-to-batch consistency, factors heavily scrutinized in GMP-compliant manufacturing. Storage recommendations typically suggest desiccated conditions at -20°C to prolong shelf life, a frequent query among laboratory managers. The compound’s compatibility with automated liquid handling systems also positions it favorably for drug discovery pipelines, where reproducibility is critical for high-content screening.

In summary, 6-Chloro-3-indolyl phosphate p-toluidine salt remains a cornerstone reagent bridging fundamental research and cutting-edge diagnostics. Its adaptability to evolving technologies like single-cell analysis and AI-driven image processing of stained samples ensures sustained relevance. As the scientific community prioritizes multiplex detection and low-abundance biomarker identification, this compound’s role will continue to expand, supported by ongoing innovations in enzymatic signal amplification strategies.

• 6578-06-9(BCIP)
• 75853-44-0(2-Acridinol,9-amino-6-chloro-, dihydrogen phosphate (ester) (9CI))
• 154201-85-1(Magenta phosphate)
• 154201-84-0(6-Chloro-3-indolyl-phosphate p-toluidine salt)
• 6769-80-8(5-BROMO-6-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE SALT)
• 1226578-81-9(6-Chloro-3-indolyl phosphate disodium salt)
• 80008-69-1(5-Bromo-3-indolyl phosphate p-toluidine salt)
• 31699-61-3(3-INDOXYL PHOSPHATE, P-TOLUIDINE SALT)
• 103404-81-5(3-INDOXYL PHOSPHATE, DI-P-TOLUIDINIUM SALT)
• 154201-83-9(1H-Indol-3-ol, 6-chloro-, dihydrogen phosphate (ester))