Mutational biosynthesis of a FK506 analogue containing a non-natural starter unit?

Molecular BioSystems Pub Date: 2012-11-28 DOI: 10.1039/C2MB25419K

Abstract

A FK506 analogue containing a non-natural starter unit was obtained through mutasynthesis by feeding cultures of Streptomyces sp. KCTC 11604BP fkbO deletion mutant with 3-cyclohexene-1-carboxylic acid. The structure of the new compound, 32-dehydroxy-FK506, and its biological activities were determined.

Graphical abstract: Mutational biosynthesis of a FK506 analogue containing a non-natural starter unit
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