Rapid fluorescence enzyme-linked immunosorbent assay for dexamethasone in equine urine
Analytical Communications Pub Date: DOI: 10.1039/AC9963300299
Abstract
A simple and sensitive competitive ELISA with a fluorescent end-point has been developed for the measurement of dexamethasone in equine urine samples. Use of pre-mixed reagent of untreated sheep antiserum and alkaline phosphatase-labelled second antibody enables the analysis of 40 duplicate urine samples within 55 min. The assay has good precision (RSD less than 10% for within and between assay runs) and is reasonably sensitive (LOD equivalent to 174 ng ml–1 of diluted equine sample). The assay has been used to detect the drug over 23 h in urine from a race horse following intramuscular injection of 20 mg of dexamethasone sodium phosphate.
Recommended Literature
- [1] Alumina grafted SBA-15 sustainable bifunctional catalysts for direct cross-coupling of benzylic alcohols to diarylmethanes? Chandran Rajendran,Govindaswamy Satishkumar,Charlotte Lang,Eric M. GaigneauxCatal. Sci. Technol., 2020,10, 2583-2592 10.1039/D0CY00471E
- [2] An artificial enzyme cascade amplification strategy for highly sensitive and specific detection of breast cancer-derived exosomes? Huiying Xu,Lu Zheng,Yu Zhou,Bang-Ce YeAnalyst, 2021,146, 5542-5549 10.1039/D1AN01071A
- [3] An atmosphere and light tuned highly diastereoselective synthesis of cyclobuta/penta[b]indoles from aniline-tethered alkylidenecyclopropanes with alkynes? Bo Cao,Yin WeiChem. Commun., 2018,54, 2870-2873 10.1039/C8CC00180D
- [4] An integrated digital microfluidic chip for multiplexed proteomic sample preparation and analysis by MALDI-MS? Hyejin Moon,Aaron R. Wheeler,Robin L. Garrell,Chang-Jin “CJ” KimLab Chip, 2006,6, 1213-1219 10.1039/B601954D
- [5] An air-stable organometallic polymer containing titanafluorene moieties obtained by the Sonogashira–Hagihara cross-coupling polycondensation? Alvin Tanudjaja,Shinsuke Inagi,Fusao Kitamura,Toshikazu Takata,Ikuyoshi TomitaDalton Trans., 2021,50, 3037-3043 10.1039/D0DT03663C
- [6] An insight into the origin of room-temperature ferromagnetism in SnO2 and Mn-doped SnO2 quantum dots: an experimental and DFT approach? Dhamodaran Manikandan,S. Amirthapandian,I. S. Zhidkov,A. I. Kukharenko,S. O. Cholakh,Ramaswamy MuruganPhys. Chem. Chem. Phys., 2018,20, 6500-6514 10.1039/C7CP07182E
- [7] An automatic determination of thoria in thoria-urania mixtures Analyst, 1966,91, 208-210 10.1039/AN9669100208
- [8] Acetylcholinesterase amperometric detection system based on a cobalt(II) tetraphenylporphyrin-modified electrode Analyst, 1996,121, 1123-1126 10.1039/AN9962101123
- [9] An integrated microfluidic platform for sensitive and rapid detection of biological toxins? Robert J. Meagher,Anson V. Hatch,Ronald F. Renzi,Anup K. SinghLab Chip, 2008,8, 2046-2053 10.1039/B815152K
- [10] An anti-ultrasonic-stripping effect in confined micro/nanoscale cavities and its applications for efficient multiscale metallic patterning? Quan Xiang,Yiqin Chen,Zhiqin Li,Kaixi Bi,Guanhua Zhang,Huigao DuanNanoscale, 2016,8, 19541-19550 10.1039/C6NR07585A
Journal Name：Analytical Communications
research_products
-
CAS no.: 89640-58-4
![1,2,3,4,5,6-Hexahydro-[2,3]bipyridinyl-6-ol](https://ossimg.kuujia.com/scimg/1271000/1270529-38-8x500.png)
![N-(3-cyanothiolan-3-yl)-2-[(2,2,2-trifluoroethyl)sulfanyl]pyridine-4-carboxamide](https://ossimg.kuujia.com/scimg/1444500/1444113-98-7x500.png)
![2,2-Difluoro-3-[methyl(2-methylbutyl)amino]propanoic acid](https://ossimg.kuujia.com/scimg/2138500/2138166-62-6x500.png)
![2-(1H-indol-3-yl)-N-{[6-(thiophen-2-yl)-[1,2,4]triazolo[4,3-b]pyridazin-3-yl]methyl}acetamide](https://ossimg.kuujia.com/scimg/2034500/2034271-14-0x500.png)