3,4,3′-Tri-O-methylellagic acid as an anticancer agent: in vitro and in silico studies?

RSC Advances Pub Date: 2022-10-19 DOI: 10.1039/D2RA05246F

Abstract

We report a natural product compound isolated from Syzygium polycephalum known as 3,4,3′-tri-O-methylellagic acid (T-EA) as a candidate drug for cancer treatment. The characterization of the isolated T-EA compound was carried out using various spectroscopic methods. The in vitro evaluation showcased the inhibition activity of T-EA towards the T47D and HeLa cell lines with EC50 values of 55.35 ± 6.28 μg mL?1 and 12.57 ± 2.22 μg mL?1, respectively. Meanwhile, the in silico evaluation aimed to understand the interaction of T-EA with enzymes responsible for cancer regulation at the molecular level by targeting the hindrance of cyclin-dependent kinase 9 (CDK9) and sirtuin 1 (SIRT1) enzymes. T-EA showed a binding free energy towards the SIRT1 protein of ΔGbind (MM-GBSA): ?30.98 ± 0.25 kcal mol?1 and ΔGbind (MM-PBSA): ?24.07 ± 0.30 kcal mol?1, while that of CDK9 was ΔGbind (MM-GBSA): ?29.50 ± 0.22 kcal mol?1 and ΔGbind (MM-PBSA): ?25.87 ± 0.40 kcal mol?1. The obtained results from this research could be considered as important information on 3,4,3′-tri-O-methylellagic acid as a drug to treat cervical and breast cancers.

Graphical abstract: 3,4,3′-Tri-O-methylellagic acid as an anticancer agent: in vitro and in silico studies
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