Residue-specific N-terminal glycine to aldehyde transformation renders analytically pure single-site labeled proteins?

Chemical Communications Pub Date: 2022-10-17 DOI: 10.1039/D2CC04196K

Abstract

Here, we present N-Gly-specific glyoxamide generation in native proteins, isolated or in a complex mixture. The resulting aldehyde enables parallel installation of probes and a purification platform to render analytically pure single-site tagged proteins. It renders N-Gly engineered insulin without perturbing its structure, receptor binding, and downstream signaling pathway.

Graphical abstract: Residue-specific N-terminal glycine to aldehyde transformation renders analytically pure single-site labeled proteins
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