Switching the chemoselectivity of perakine reductase for selective reduction of α,β-unsaturated ketones by Arg127 mutation?
Chemical Communications Pub Date: 2023-05-23 DOI: 10.1039/D3CC00850A
Abstract
The chemoselectivity of perakine reductase (PR) was engineered through rational design. We identified Arg127 as a control site of chemoselectivity. Mutation of Arg127 switched the chemoselectivity of PR between C![[double bond, length as m-dash]](https://www.rsc.org/images/entities/char_e001.gif)
O and CC or led to non-selectivity towards α,β-unsaturated ketones, leading to the production of allylic alcohols, saturated ketones, or a mixture of both. This study provides an example for developing novel reductases for α,β-unsaturated ketones.
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Journal Name：Chemical Communications
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CAS no.: 89640-58-4
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