Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flumetralin in orange?

Analyst Pub Date: 2022-06-30 DOI: 10.1039/D2AN00899H

Abstract

Residues of flumetralin, a dinitroaniline herbicide, in foods pose a health risk to humans. In this study, we produced a sensitive monoclonal antibody (mAb) against flumetralin and developed a gold nanoparticle-based lateral flow immunoassay (LFIA) strip for screening flumetralin in orange. The subtype of mAb 3F5 was IgG1 and had a half-maximal inhibitory concentration of 9.43 ng mL?1. The cross-reactivity assay indicated that mAb had good specificity. The LFIA strip had a visual limit of detection of 200 ng g?1 in orange. We obtained results with the naked eye within 15 min. The calculated LOD of the strip in orange was 33.26 ng g?1, with a linear detection of 80.72–1672.10 ng g?1. The strip had recovery rates ranging from 98.4% to 110.1%. Therefore, our developed method is an effective and reliable tool for the rapid detection of flumetralin in orange.

Graphical abstract: Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flumetralin in orange
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