Analytical-scale purification of trichostatin A from bacterial culture in a single step and with high selectivity using immobilised metal affinity chromatography

RSC Advances Pub Date: 2011-11-08 DOI: 10.1039/C1RA00864A

Abstract

The potent histone deacetylase inhibitor trichostatin A (TSA) has been captured in a single step with high selectivity from the culture supernatant of the native TSA-producing strain Streptomyces hygroscopicusMST-AS5346 using analytical-scale Ni(II)-based immobilised metal affinity chromatography.

Graphical abstract: Analytical-scale purification of trichostatin A from bacterial culture in a single step and with high selectivity using immobilised metal affinity chromatography
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