Reversible clustering of magnetic nanobiocatalysts for high-performance biocatalysis and easy catalyst recycling?

Chemical Communications Pub Date: 2012-03-08 DOI: 10.1039/C2CC30953J

Abstract

Reversible clusters of nanobiocatalysts are developed via non-covalent interaction among enzyme-bound iron oxide magnetic nanoparticles. Dissociation of the clusters by shaking during biotransformation enables high catalytic performance, and re-clustering by stopping shaking after reaction allows for easy magnetic separation. The novel concept is demonstrated with alcohol dehydrogenase RDR for the enantioselective reduction of 7-methoxy-2-tetralone.

Graphical abstract: Reversible clustering of magnetic nanobiocatalysts for high-performance biocatalysis and easy catalyst recycling
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