Rapid identification of new bacterial alcohol dehydrogenases for (R)- and (S)-enantioselective reduction of ?-ketoesters?

Chemical Communications Pub Date: 2004-08-06 DOI: 10.1039/B407004F

Abstract

New bacterial alcohol dehydrogenases with high and complementary enantioselectivity for the reduction of ethyl 3-keto-4,4,4-trifluorobutyrate 1 and methyl 3-keto-3-(3′-pyridyl)-propionate 3 have been rapidly identified by use of a new methodology consisting of preselection of microorganisms based on degradation ability and high-throughput screening with a miniaturized system coupled with fast analysis of enantioselectivities.

Graphical abstract: Rapid identification of new bacterial alcohol dehydrogenases for (R)- and (S)-enantioselective reduction of ?-ketoesters
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