An improved fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran: its origin and application?

Chemical Communications Pub Date: 2013-10-17 DOI: 10.1039/C3CC47264G

Abstract

We have developed a fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran. The reaction of NAD(P)+ with 2-acetylbenzofuran produced a fluorescent product, allowing the highly-sensitive and quick detection of NAD(P)+. This method was successfully applied to the detection of P450 substrates in the microtiter-plate format.

Graphical abstract: An improved fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran: its origin and application
Recommended Literature