Multi-way calibration coupling with fluorescence spectroscopy to determine magnolol and honokiol in herb and plasma samples

Analytical Methods Pub Date: 2015-06-11 DOI: 10.1039/C5AY01285F

Abstract

This work presents a novel approach for the simultaneous determination of mixtures of magnolol and honokiol in herb and plasma samples by combining the sensitivity of molecular fluorescence and the selectivity of the second-order calibration method. The excitation–emission matrix fluorescence data were processed by applying the second-order calibration method based on parallel factor analysis (PARAFAC) and self-weighted alternating trilinear decomposition (SWATLD) algorithms. The results showed that the method could solve the problem of analyzing complex multi-component samples, using “mathematics separation” to replace some or enhance chemical separation. The recoveries from spiked herb samples were in the range of 93–104% for magnolol and 89–98% for honokiol, and those from spiked human plasma samples were in the range of 98–105% for magnolol and 94–96% for honokiol. In herb samples, the LOD values of magnolol were 0.51 μg ml?1 and 0.45 μg ml?1, and those of honokiol were 0.37 μg ml?1 and 0.24 μg ml?1, when using PARAFAC and SWATLD. For human plasma samples, the LOD values of magnolol were 0.93 μg ml?1 and 0.64 μg ml?1, and 0.79 μg ml?1 and 0.42 μg ml?1 for honokiol. The results demonstrated that this method had both high recovery and good precision for the determination of honokiol and magnolol. The proposed method avoided preconcentration and had a simple disposal process, so it considerably decreased the analysis time and the experimental costs. Thus, it can be considered as a green analytical procedure for magnolol and honokiol determination in herb and plasma samples.

Graphical abstract: Multi-way calibration coupling with fluorescence spectroscopy to determine magnolol and honokiol in herb and plasma samples
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