Microwave-assisted Kochetkov amination followed by permanent charge derivatization: a facile strategy for glycomics?

Chemical Communications Pub Date: 2010-09-08 DOI: 10.1039/C0CC01732A

Abstract

We report a simple and rapid microwave-assisted method for the preparation of oligosaccharide-glycosylamines, followed by labelling with tris(2,4,6-trimethoxyphenyl)phosphonium acetic acid N-hydroxysuccinimide ester. The facile strategy introduced a permanent charge at the reducing end of the oligosaccharide. In combination of MALDI-MS, the detection limit for maltoheptaose was as low as 2 fmol μL?1.

Graphical abstract: Microwave-assisted Kochetkov amination followed by permanent charge derivatization: a facile strategy for glycomics
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