4-Phosphothiophen-2-yl alanine: a new 5-membered analogue of phosphotyrosine?

Chemical Communications Pub Date: 2014-06-30 DOI: 10.1039/C4CC03393K

Abstract

Polyclonal antibodies raised against 4-phosphothiophen-2-yl alanine 2a, a novel five-membered ring analogue of phosphotyrosine, showed high selectivity for phosphotyrosine and no cross-reactivity with other phosphorylated amino acids. Western blots showed that the polyclonal was similarly effective, but different in selectivity, to a commercially available monoclonal antibody.

Graphical abstract: 4-Phosphothiophen-2-yl alanine: a new 5-membered analogue of phosphotyrosine
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