Intriguing cellular processing of a fluorinated amino acid during protein biosynthesis in Escherichia coli?
Organic & Biomolecular Chemistry Pub Date: 2016-09-08 DOI: 10.1039/C6OB01690A
Abstract
Bioincorporation of the methionine analogue S-(2-fluoroethyl)-L-homocysteine (L-MFE) into bacteriophage lysozyme overproduced in Escherichia coli results not only in the expected L-MFE incorporation but surprisingly substantial L-vinthionine incorporation into the labeled lysozymes. Synthetic L-vinthionine itself however is not activated by purified Escherichia coli methionyl-tRNA synthetase. The indirect preparation of vinthionine-containing proteins has the potential to be an alternate strategy to prepare vinyl thioether moieties for click chemistry applications on proteins.
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Journal Name：Organic & Biomolecular Chemistry
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CAS no.: 89640-58-4
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