Ultrasensitive detection of seventeen chemicals simultaneously using paper-based sensors?

Materials Chemistry Frontiers Pub Date: 2018-07-30 DOI: 10.1039/C8QM00336J

Abstract

We developed an ultrasensitive gold nanoparticle-based multicomponent lateral-flow strip assay for the simultaneous detection of seventeen hormone drugs from three classes: nandrolone (NR) and its analogues, dexamethasone (DEX) and its analogues, and hexestrol (HES) and its analogues. The antibodies and coating antigens were selected for the assay and the multi-immunochromatographic strip (multi-ICS) exhibited a very low limit of detection (LOD). Quantitative results were obtained using a strip scan reader, giving LOD values of 0.06–4.85 ng mL?1 for NR and its analogues, 0.005–2.85 ng mL?1 for DEX and its analogues, and 0.03–2.14 ng mL?1 for HES and its analogues. The recovery rates from milk samples were 85.8–93.3% for NR, 78.8–87.5% for DEX, and 89.4–99.4% for HES. This multi-ICS assay therefore provides a useful tool for on-site detection and rapid initial screening of hormone drugs in biological samples.

Graphical abstract: Ultrasensitive detection of seventeen chemicals simultaneously using paper-based sensors
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