The spectrophotometric determination of Ampicillin

ANALYSTANALYST Pub Date: DOI: 10.1039/AN9679200247

Abstract

A method is described for the determination of Ampicillin (α-amino-benzyl penicillin) based on the spectrophotometric measurement at 320 mμ of a compound formed by acid degradation of the penicillin at 75° C in the presence of buffer solution at pH 5·2 and a trace of a copper salt. Results obtained on samples of Ampicillin, Ampicillin Trihydrate and Ampicillin Sodium show good agreement with those recorded by the cup-plate microbiological method.

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