Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells?
RSC Advances Pub Date: 2018-12-19 DOI: 10.1039/C8RA09303B
Abstract
We developed a fast strain-promoted azide–alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M?1 s?1, which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials.
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Journal Name：RSC Advances
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CAS no.: 89640-58-4
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