Single-cell-resolved measurement of enzyme activity at the tissue level using drop-on-demand microkits?
Analyst Pub Date: 2020-12-22 DOI: 10.1039/D0AN02247K
Abstract
Drop-on-demand microkits with a diameter of ～20 μm are used to measure the activity of acetylcholinesterase (AChE) in a brain slice with single-cell resolution. The relative standard deviation from 25 cellular regions reached 73.3% exhibiting the difference of enzyme activity in the brain slice. Therefore, this approach utilizing the well-established kits provides an alternative single-cell-resolved strategy for the elucidation of enzymatic heterogeneity at the tissue level.
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Journal Name：Analyst
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CAS no.: 89640-58-4
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