Quenching of the long-lived Ru(ii)bathophenanthroline luminescence for the detection of supramolecular interactions

Organic & Biomolecular Chemistry Pub Date: 2008-04-28 DOI: 10.1039/B800357B

Abstract

A feasibility study based on tailor-made peptide sequences for a new robust luminescence probe-system using the long-lived luminescence of a Ru(II)-bathophenanthroline complex in combination with an efficient anthraquinone-type quencher is presented. Due do their high chemical stability, both dyes can be introduced during solid-phase peptide synthesis avoiding post-synthetic labelling. Photophysical measurements revealed an intense quenching of the luminescence of the Ru-complex (65–68%) which was also confirmed by calculations resulting from decay time measurements. The long-lived luminescence allows for a time-gated detection scheme, which can reduce any luminescence contribution from matrix components.

Graphical abstract: Quenching of the long-lived Ru(ii)bathophenanthroline luminescence for the detection of supramolecular interactions
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