Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)?
Chemical Communications Pub Date: 2014-02-28 DOI: 10.1039/C4CC00540F
Abstract
We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples.
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Journal Name:Chemical Communications
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CAS no.: 89640-58-4